CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015366
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras-related C3 botulinum toxin substrate 1 
Protein Synonyms/Alias
 p21-Rac1 
Gene Name
 Rac1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
123KLDLRDDKDTIEKLKubiquitination[1]
128DDKDTIEKLKEKKLTubiquitination[1]
133IEKLKEKKLTPITYPubiquitination[1]
147PQGLAMAKEIGAVKYacetylation[2]
153AKEIGAVKYLECSALacetylation[2]
166ALTQRGLKTVFDEAIubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
 Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (By similarity). Stimulates PKN2 kinase activity (By similarity). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. 
Sequence Annotation
 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 115 118 GTP (By similarity).
 MOTIF 32 40 Effector region (Potential).
 MOD_RES 189 189 Cysteine methyl ester (By similarity).
 LIPID 189 189 S-geranylgeranyl cysteine (By
 CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; GTP-binding; Isopeptide bond; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 192 AA 
Protein Sequence
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG 60
QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR 120
DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP 180
PVKKRKRKCL LL 192 
Gene Ontology
 GO:0031410; C:cytoplasmic vesicle; IDA:RGD.
 GO:0019897; C:extrinsic to plasma membrane; IEA:Compara.
 GO:0000139; C:Golgi membrane; IDA:RGD.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0001891; C:phagocytic cup; IEA:Compara.
 GO:0032587; C:ruffle membrane; IEA:Compara.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0005525; F:GTP binding; IDA:RGD.
 GO:0003924; F:GTPase activity; IDA:RGD.
 GO:0007015; P:actin filament organization; IMP:RGD.
 GO:0048532; P:anatomical structure arrangement; IEA:Compara.
 GO:0002093; P:auditory receptor cell morphogenesis; IEA:Compara.
 GO:0007411; P:axon guidance; IEA:Compara.
 GO:0045453; P:bone resorption; IDA:RGD.
 GO:0008283; P:cell proliferation; IMP:RGD.
 GO:0045216; P:cell-cell junction organization; IEA:Compara.
 GO:0021799; P:cerebral cortex radially oriented cell migration; IEA:Compara.
 GO:0090103; P:cochlea morphogenesis; IEA:Compara.
 GO:0048813; P:dendrite morphogenesis; IEA:Compara.
 GO:0071542; P:dopaminergic neuron differentiation; IEA:Compara.
 GO:0021831; P:embryonic olfactory bulb interneuron precursor migration; IEA:Compara.
 GO:0043652; P:engulfment of apoptotic cell; IEA:Compara.
 GO:0003382; P:epithelial cell morphogenesis; IEA:Compara.
 GO:0006972; P:hyperosmotic response; IEA:Compara.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0051668; P:localization within membrane; IEA:Compara.
 GO:0002551; P:mast cell chemotaxis; IMP:RGD.
 GO:0032707; P:negative regulation of interleukin-23 production; IEA:Compara.
 GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
 GO:0045740; P:positive regulation of DNA replication; IMP:RGD.
 GO:0010592; P:positive regulation of lamellipodium assembly; IEA:Compara.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Compara.
 GO:0016601; P:Rac protein signal transduction; TAS:RGD.
 GO:0030334; P:regulation of cell migration; ISS:UniProtKB.
 GO:0060263; P:regulation of respiratory burst; IEA:Compara.
 GO:0097178; P:ruffle assembly; IEA:Compara.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Compara.
 GO:0060071; P:Wnt receptor signaling pathway, planar cell polarity pathway; IEA:Compara. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 
Pfam
 PF00071; Ras 
SMART
 SM00174; RHO 
PROSITE
 PS51420; RHO 
PRINTS
 PR00449; RASTRNSFRMNG.