CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023941
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting nexin-9 
Protein Synonyms/Alias
 SH3 and PX domain-containing protein 1; Protein SDP1; SH3 and PX domain-containing protein 3A 
Gene Name
 SNX9 
Gene Synonyms/Alias
 SH3PX1; SH3PXD3A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
179PKSSSYFKDSESADAubiquitination[1, 2]
207SMKIPLNKFPGFAKPubiquitination[3]
213NKFPGFAKPGTEQYLubiquitination[3]
223TEQYLLAKQLAKPKEubiquitination[4, 5]
267GSKMYGLKSYIEYQLubiquitination[1]
288RSVNHRYKHFDWLYEacetylation[6]
288RSVNHRYKHFDWLYEubiquitination[3]
300LYERLLVKFGSAIPIubiquitination[1, 2]
313PIPSLPDKQVTGRFEubiquitination[1, 2, 3, 7]
325RFEEEFIKMRMERLQubiquitination[1, 3]
360FLNFRDEKEWKTGKRubiquitination[2, 8]
407EAVGKFTKAMDDGVKubiquitination[1, 2, 3]
414KAMDDGVKELLTVGQubiquitination[1, 2, 3]
425TVGQEHWKRCTGPLPubiquitination[3]
440KEYQKIGKALQSLATubiquitination[1, 2]
483SLVAEQPKKDLHFLMubiquitination[1]
511PDIIGTHKGAIEKVKubiquitination[3]
528DKLVATSKITLQDKQubiquitination[1]
534SKITLQDKQNMVKRVubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F- actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate. 
Sequence Annotation
 DOMAIN 1 62 SH3.
 DOMAIN 250 361 PX.
 DOMAIN 392 595 BAR.
 REGION 201 213 Critical for tubulation activity.
 BINDING 286 286 Phosphatidylinositol 4,5-bisphosphate.
 BINDING 288 288 Phosphatidylinositol 4,5-bisphosphate.
 BINDING 313 313 Phosphatidylinositol 4,5-bisphosphate.
 BINDING 327 327 Phosphatidylinositol 4,5-bisphosphate.
 MOD_RES 176 176 Phosphoserine (By similarity).
 MOD_RES 288 288 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; SH3 domain; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 595 AA 
Protein Sequence
MATKARVMYD FAAEPGNNEL TVNEGEIITI TNPDVGGGWL EGRNIKGERG LVPTDYVEIL 60
PSDGKDQFSC GNSVADQAFL DSLSASTAQA SSSAASNNHQ VGSGNDPWSA WSASKSGNWE 120
SSEGWGAQPE GAGAQRNTNT PNNWDTAFGH PQAYQGPATG DDDDWDEDWD GPKSSSYFKD 180
SESADAGGAQ RGNSRASSSS MKIPLNKFPG FAKPGTEQYL LAKQLAKPKE KIPIIVGDYG 240
PMWVYPTSTF DCVVADPRKG SKMYGLKSYI EYQLTPTNTN RSVNHRYKHF DWLYERLLVK 300
FGSAIPIPSL PDKQVTGRFE EEFIKMRMER LQAWMTRMCR HPVISESEVF QQFLNFRDEK 360
EWKTGKRKAE RDELAGVMIF STMEPEAPDL DLVEIEQKCE AVGKFTKAMD DGVKELLTVG 420
QEHWKRCTGP LPKEYQKIGK ALQSLATVFS SSGYQGETDL NDAITEAGKT YEEIASLVAE 480
QPKKDLHFLM ECNHEYKGFL GCFPDIIGTH KGAIEKVKES DKLVATSKIT LQDKQNMVKR 540
VSIMSYALQA EMNHFHSNRI YDYNSVIRLY LEQQVQFYET IAEKLRQALS RFPVM 595 
Gene Ontology
 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0005545; F:1-phosphatidylinositol binding; IDA:UniProtKB.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IMP:UniProtKB.
 GO:0060988; P:lipid tube assembly; IDA:UniProtKB.
 GO:0043547; P:positive regulation of GTPase activity; IDA:UniProtKB.
 GO:0032461; P:positive regulation of protein oligomerization; IDA:UniProtKB.
 GO:0006898; P:receptor-mediated endocytosis; IMP:UniProtKB. 
Interpro
 IPR001683; Phox.
 IPR001452; SH3_domain.
 IPR014536; Snx9.
 IPR019497; Sorting_nexin_WASP-bd-dom. 
Pfam
 PF10456; BAR_3_WASP_bdg
 PF00787; PX
 PF00018; SH3_1 
SMART
 SM00312; PX
 SM00326; SH3 
PROSITE
 PS51021; BAR
 PS50195; PX
 PS50002; SH3 
PRINTS