UniProt Accession

 MYL3_HUMAN; P08590; B2R534; Q9NRS8 

Genbank Protein ID

 M24122; X07373; M24247; M24242; M24243; M24244; M24245; M24246; AF174483; AK312044; CH471055; BC009790 

Genbank Nucleotide ID

 AAA59895.1; CAA30292.1; AAA59851.1; AAA59851.1; AAA59851.1; AAA59851.1; AAA59851.1; AAA59851.1; AAF91089.1; BAG34981.1; EAW64791.1; AAH09790.1 

Protein Name

 Myosin light chain 3 

Protein Synonyms/Alias

 Cardiac myosin light chain 1; CMLC1; Myosin light chain 1, slow-twitch muscle B/ventricular isoform; MLC1SB; Ventricular/slow twitch myosin alkali light chain 

Gene Name

 MYL3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
142	EGLRVFDKEGNGTVM	ubiquitination	[1, 2, 3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Regulatory light chain of myosin. Does not bind calcium. 

Sequence Annotation

 DOMAIN 49 86 EF-hand 1.
 DOMAIN 128 163 EF-hand 2.
 DOMAIN 163 195 EF-hand 3.
 MOD_RES 2 2 N,N,N-trimethylalanine (Probable).  

Keyword

 Cardiomyopathy; Complete proteome; Direct protein sequencing; Disease mutation; Methylation; Motor protein; Muscle protein; Myosin; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 195 AA 

Protein Sequence

Gene Ontology

 GO:0031672; C:A band; IDA:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031674; C:I band; IDA:BHF-UCL.
 GO:0005859; C:muscle myosin complex; TAS:ProtInc.
 GO:0003785; F:actin monomer binding; IDA:BHF-UCL.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003774; F:motor activity; IEA:Compara.
 GO:0032038; F:myosin II heavy chain binding; NAS:BHF-UCL.
 GO:0008307; F:structural constituent of muscle; TAS:ProtInc.
 GO:0060048; P:cardiac muscle contraction; IMP:BHF-UCL.
 GO:0030049; P:muscle filament sliding; TAS:Reactome.
 GO:0032781; P:positive regulation of ATPase activity; ISS:BHF-UCL.
 GO:0006942; P:regulation of striated muscle contraction; IMP:BHF-UCL.
 GO:0002026; P:regulation of the force of heart contraction; IMP:BHF-UCL.
 GO:0007519; P:skeletal muscle tissue development; IEA:Compara.
 GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; IMP:BHF-UCL. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

  

SMART

  

PROSITE

 PS50222; EF_HAND_2 

PRINTS