UniProt Accession

 RSC8_YEAST; P43609; D6VTS0; Q6B289 

Genbank Protein ID

 D50617; AY692841; BK006940 

Genbank Nucleotide ID

 BAA09276.1; AAT92860.1; DAA12480.1 

Protein Name

 Chromatin structure-remodeling complex protein RSC8 

Protein Synonyms/Alias

 Remodel the structure of chromatin complex subunit 8; SWI3 homolog 

Gene Name

 RSC8 

Gene Synonyms/Alias

 SWH3; YFR037C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
232	PVNLTIKKNVYDSAQ	ubiquitination	[1]
375	VGSTLNGKGGDSRDG	ubiquitination	[1]
439	LVKLTMEKLESKFTK	ubiquitination	[1]
446	KLESKFTKLCDLETQ	ubiquitination	[1]
458	ETQLEMEKLKYVKES	ubiquitination	[1]
529	TATGHGVKRVGKEGE	acetylation	[2]
553	KLQPQVYKPWSL***	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression. 

Sequence Annotation

 DOMAIN 80 177 SWIRM.
 DOMAIN 310 362 SANT.
 ZN_FING 254 298 ZZ-type.
 MOD_RES 485 485 Phosphoserine.  

Keyword

 Chromatin regulator; Coiled coil; Complete proteome; Direct protein sequencing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 557 AA 

Protein Sequence

Gene Ontology

 GO:0016586; C:RSC complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043044; P:ATP-dependent chromatin remodeling; IDA:UniProtKB.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:SGD.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:SGD.
 GO:0006337; P:nucleosome disassembly; IDA:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:SGD. 

Interpro

 IPR009057; Homeodomain-like.
 IPR001005; SANT/Myb.
 IPR017884; SANT_dom.
 IPR007526; SWIRM.
 IPR011991; WHTH_DNA-bd_dom.
 IPR000433; Znf_ZZ. 

Pfam

 PF00249; Myb_DNA-binding
 PF04433; SWIRM
 PF00569; ZZ 

SMART

 SM00717; SANT
 SM00291; ZnF_ZZ 

PROSITE

 PS51293; SANT
 PS50934; SWIRM
 PS01357; ZF_ZZ_1
 PS50135; ZF_ZZ_2 

PRINTS