CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004368
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-isopropylmalate dehydratase large subunit 1 
Protein Synonyms/Alias
 Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 
Gene Name
 leuC1 
Gene Synonyms/Alias
 leuC; STM0111 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
8MAKTLYEKLFDAHVVacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity). 
Sequence Annotation
 METAL 347 347 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 407 407 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 410 410 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Iron; Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 466 AA 
Protein Sequence
MAKTLYEKLF DAHVVFEAPN ETPLLYIDRH LVHEVTSPQA FDGLRAHHRP VRQPGKTFAT 60
MDHNVSTQTK DINASGEMAR IQMQELIKNC NEFGVELYDL NHPYQGIVHV MGPEQGVTLP 120
GMTIVCGDSH TATHGAFGAL AFGIGTSEVE HVLATQTLKQ GRAKTMKIEV TGNAAPGITA 180
KDIVLAIIGK TGSAGGTGHV VEFCGDAIRA LSMEGRMTLC NMAIEMGAKA GLVAPDETTF 240
NYVKGRLHAP KGRDFDEAVE YWKTLKTDDG ATFDTVVALR AEEIAPQVTW GTNPGQVISV 300
TDIIPDPASF SDPVERASAE KALAYMGLQP GVPLTDVAID KVFIGSCTNS RIEDLRAAAE 360
VAKGRKVAPG VQALVVPGSG PVKAQAEAEG LDKIFIEAGF EWRLPGCSMC LAMNNDRLNP 420
GERCASTSNR NFEGRQGRGG RTHLVSPAMA AAAAVTGHFA DIRSIK 466 
Gene Ontology
 GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009098; P:leucine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR004430; 3-IsopropMal_deHydase_lsu.
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS. 
Pfam
 PF00330; Aconitase 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.