CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031266
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SEC11-like 1 (S. cerevisiae), isoform CRA_d 
Protein Synonyms/Alias
 Signal peptidase complex catalytic subunit SEC11A; cDNA FLJ51313, highly similar to Microsomal signal peptidase 18 kDa subunit(EC 3.4.-.-) 
Gene Name
 SEC11A 
Gene Synonyms/Alias
 SEC11L1; hCG_26624 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
74PIVHRVLKIHEKQNGubiquitination[1, 2]
78RVLKIHEKQNGHIKFubiquitination[1, 3, 4]
84EKQNGHIKFLTKGDNubiquitination[1, 2]
88GHIKFLTKGDNNAVDubiquitination[1, 2, 3, 4, 5, 6, 7]
101VDDRGLYKQGQHWLEubiquitination[1, 2, 3, 5, 7]
109QGQHWLEKKDVVGRAubiquitination[1, 2, 3]
110GQHWLEKKDVVGRARubiquitination[1, 2]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Membrane; Protease; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 153 AA 
Protein Sequence
MIVSSALMIW KGLMVITGSE SPIVVVLSGS MEPAFHRGDL LFLTNRVEDP IRVGEIVVFR 60
IEGREIPIVH RVLKIHEKQN GHIKFLTKGD NNAVDDRGLY KQGQHWLEKK DVVGRARGFV 120
PYIGIVTILM NDYPKFKYAV LFLLGLFVLV HRE 153 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0008236; F:serine-type peptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006465; P:signal peptide processing; IEA:InterPro. 
Interpro
 IPR019758; Pept_S26A_signal_pept_1_CS.
 IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
 IPR019759; Peptidase_S24_S26.
 IPR015927; Peptidase_S24_S26A/B/C.
 IPR011056; Peptidase_S24_S26A/B/C_b-rbn.
 IPR001733; Peptidase_S26B. 
Pfam
 PF00717; Peptidase_S24 
SMART
  
PROSITE
 PS00501; SPASE_I_1
 PS00761; SPASE_I_3 
PRINTS
 PR00728; SIGNALPTASE.