CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037153
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Microtubule-associated protein 
Protein Synonyms/Alias
  
Gene Name
 MAP4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
286KDMALATKTEVALAKubiquitination[1]
329DMELPTEKEVALVKDubiquitination[1]
349ETDVSSAKNVVLPTEubiquitination[2]
363ETEVAPAKDVTLLKEacetylation[3]
363ETEVAPAKDVTLLKEubiquitination[2, 4]
385KMDLAPSKDMGPPKEacetylation[3, 5, 6]
385KMDLAPSKDMGPPKEubiquitination[4]
481NVILTKDKALPLEAEubiquitination[2]
493EAEVAPVKDMAQLPEubiquitination[2]
507ETEIAPAKDVAPSTVubiquitination[2]
515DVAPSTVKEVGLLKDacetylation[3]
515DVAPSTVKEVGLLKDubiquitination[1, 4]
577KTEAPLAKDGVLTLAubiquitination[2]
591ANNVTPAKDVPPLSEubiquitination[2]
1871TQPTSLPKQPAPTTIubiquitination[1]
2078NTSAPDLKNVRSKVGubiquitination[1]
2091VGSTENIKHQPGGGRubiquitination[1]
2215FKEKAQAKVGSLDNVubiquitination[1]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Microtubule; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2297 AA 
Protein Sequence
MADLSLADAL TEPSPDIEGE IKRDFIATLE AEAFDDVVGE TVGKTDYIPL LDVDEKTGNS 60
ESKKKPCSET SQIEDLPLPP HPASLSFHLP LDTPSSKPTL LANGGHGVEG SDTTGSPTEF 120
LEEKMAYQEY PNSQNWPEDT NFCFQPEQVV DPIQTDPFKM YHDDDLADLV FPSSATADTS 180
IFAGQNDPLK DSYGMSPCNT AVVPQGWSVE ALNSPHSESF VSPEAVAEPP QPTAVPLELA 240
KEIEMASEER PPAQALEIMM GLKTTDMAPS KETEMALAKD MALATKTEVA LAKDMESPTK 300
LDVTLAKDMQ PSMESDMALV KDMELPTEKE VALVKDVRWP TETDVSSAKN VVLPTETEVA 360
PAKDVTLLKE TERASPIKMD LAPSKDMGPP KENKKETERA SPIKMDLAPS KDMGPPKENK 420
IVPAKDLVLL SEIEVAQAND IISSTEISSA EKVALSSETE VALARDMTLP PETNVILTKD 480
KALPLEAEVA PVKDMAQLPE TEIAPAKDVA PSTVKEVGLL KDMSPLSETE MALGKDVTPP 540
PETEVVLIKN VCLPPEMEVA LTEDQVPALK TEAPLAKDGV LTLANNVTPA KDVPPLSETE 600
ATPVPIKDME IAQTQKGISE DSHLESLQDV GQSAAPTFMI SPETVTGTGK KCSLPAEEDS 660
VLEKLGERKP CNSQPSELSS ETSANFMYCG TPPTQAKQVC RPSDRRSTRP KPARVPPELL 720
GGSPPWKTLD HRLGHCSLSE SGWVSGSSSC GGPGNQRKSI HVDSLEPQRD LGREAWDIES 780
TPIMMKKKKK KPKQKRYSQP RAGGPSDDDN ADKPKGHPFA ADTQKSGVLP SQPTTMGTEY 840
GLVSGENLKR ECLVNSSAAR LVAENFVSES LRIPLYPSEE APKTAISSQS KLRVEEESKS 900
NKSVLQNQDK KLLKQHEYKP QPAPHLKTPV DKSQSVGPLN LKGPLAEVSA YNVETPLDIR 960
LKEGCSPFLD QEVMGVVSKP TAAKEIPNLV PTLIASNPLE CNLKEGNNES KMTKLQNVKL 1020
KEFPEGAEED KELKKEAFPN ERQEISIFTS EQLQGQVLVQ VPGVENEPFK RMAGDGKSRK 1080
GRGSSGKMRT DSGKVKAKSE LPFLLDSQKD GRAVLIPSEP VSKTEGMTTQ DKSEELGLNS 1140
SKQPGTKADL TEAVVMGEPK EMTQPKVAGT MQALIPLESG SGMTQTSGVS TETGDVVKDM 1200
GVNNQSKEGR CPWKDHEAAP WISEKPKKRG NEGKSKKFKN NYSTQPARME RKEEILNPPF 1260
EGKDGDTGSI PHKSKEIGFT FPKMHDSSFS HTPDTPTVEA VDRKGGNFQV NFVELGTLGE 1320
NKISTVKAST VTEPPAKVTD VSCQEQIQGA GFVPSVVSEE NKTDAANRYT AVADKPSKRS 1380
NDGKSKKVKN SSPEKHILEN KIDATKIHVP METTGDQGIE GMAYMDENRN ITFTCPRTPS 1440
ELINKSSPLE VLESAACEKL PTPTPQVVKE GDSFPDTLAK NGQEIAPAQI SKSLMVDNYT 1500
KDGVPGQERP KGPSAVVPST STGGVALPIT TAIETVNIHG DHSLKNKAEL ADSMKNEAGI 1560
DEGHVIGESE SVHSGASKHS VEKVTELAKG HLLPGVPVED QSLPGEARAL EGYADRGNFP 1620
AHPVNEEKET KEGSVAVQIP DLLEDKAQKL SFCEDQNAQD RNSKGSDSLN KKVDLTLLSP 1680
KSENDKLKEI SLACKITELE SVSLPTPEIQ SDFLHSKVEA PPSEVADTLV IMTASKGVRL 1740
PEPKDKILET PQKMTEKSES KTPGEGKKED KSRMAEPMKG YMRPTKSRGL TPLLPKSTIQ 1800
EQERHKQLKS AGIARPEEGR PVVSGTGNDI TTPPNKELPP SPEKKTKPLA TTQPAKTSTS 1860
KAKTQPTSLP KQPAPTTIGG LNKKPMSLAS GLVPAAPPKR PAVASARPSI LPSKDVKPKP 1920
IADAKAPEKR ASPSKPASAP ASRSGSKSTQ TVAKTTTAAA VASTGPSSRS PSTLLPKKPT 1980
AIKTEGKPAE VKKMTAKSVP ADLSRPKSTS TSSMKKTTTL SGTAPAAGVV PSRVKATPMP 2040
SRPSTTPFID KKPTSAKPSS TTPRLSRLAT NTSAPDLKNV RSKVGSTENI KHQPGGGRAK 2100
VEKKTEAAAT TRKPESNAVT KTAGPIASAQ KQPAGKVQIV SKKVSYSHIQ SKCGSKDNIK 2160
HVPGGGNVQI QNKKVDISKV SSKCGSKANI KHKPGGGDVK IESQKLNFKE KAQAKVGSLD 2220
NVGHLPAGGA VKTEGGGSEA PLCPGPPAGE EPAISEAAPE AGAPTSASGL NGHPTLSGGG 2280
DQREAQTLDS QIQETSI 2297 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA. 
Interpro
 IPR027324; MAP2/MAP4/Tau.
 IPR027323; MAP4.
 IPR001084; Tau/MAP_tubulin-bd_rpt. 
Pfam
 PF00418; Tubulin-binding 
SMART
  
PROSITE
 PS00229; TAU_MAP_1
 PS51491; TAU_MAP_2 
PRINTS