UniProt Accession

 BIOH_ECOLI; P13001; Q2M777 

Genbank Protein ID

 X15587; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA33612.1; AAA58210.1; AAC76437.1; BAE77879.1 

Protein Name

 Pimelyl-[acyl-carrier protein] methyl ester esterase 

Protein Synonyms/Alias

 Biotin synthesis protein BioH; Carboxylesterase BioH 

Gene Name

 bioH 

Gene Synonyms/Alias

 bioB; b3412; JW3375 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
220	KVVPMLDKLWPHSES	acetylation	[1]
253	CHLLVALKQRV****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. E.coli employs a methylation and demethylation strategy to allow elongation of a temporarily disguised malonate moiety to a pimelate moiety by the fatty acid synthetic enzymes. BioH shows a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons) and short chain p-nitrophenyl esters. Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic acid into pimeloyl-CoA, a precursor in biotin biosynthesis. 

Sequence Annotation

 REGION 82 83 Substrate binding.
 REGION 143 147 Substrate binding.
 ACT_SITE 82 82 Nucleophile.
 ACT_SITE 207 207 By similarity.
 ACT_SITE 235 235
 BINDING 22 22 Substrate; via amide nitrogen and
 BINDING 235 235 Substrate.  

Keyword

 3D-structure; Biotin biosynthesis; Complete proteome; Cytoplasm; Hydrolase; Reference proteome; Serine esterase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 256 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004091; F:carboxylesterase activity; IDA:UniProtKB.
 GO:0090499; F:pimelyl-[acyl-carrier protein] methyl ester esterase activity; IDA:EcoCyc.
 GO:0009102; P:biotin biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR000073; AB_hydrolase_1.
 IPR010076; BioH. 

Pfam

 PF00561; Abhydrolase_1 

SMART

  

PROSITE

  

PRINTS