CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024640
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 4 
Protein Synonyms/Alias
 Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4 
Gene Name
 Usp4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
835YNRYWRDKLDTVVEFubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. 
Sequence Annotation
 DOMAIN 11 122 DUSP.
 DOMAIN 142 226 Ubiquitin-like 1.
 DOMAIN 483 571 Ubiquitin-like 2.
 REGION 405 407 Necessary for interaction with RBL2 (By
 REGION 459 463 Necessary for interaction with RB1 and
 MOTIF 133 141 Nuclear export signal (By similarity).
 MOTIF 765 770 Nuclear localization signal (By
 ACT_SITE 311 311 By similarity.
 ACT_SITE 879 879 By similarity.
 METAL 461 461 Zinc (By similarity).
 METAL 464 464 Zinc (By similarity).
 METAL 797 797 Zinc (By similarity).
 METAL 800 800 Zinc (By similarity).
 MOD_RES 679 679 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nucleus; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation; Ubl conjugation pathway; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 961 AA 
Protein Sequence
MAEGRGTHER PDVETQKTEL GALMGTTLQR GAQWYLIDSR WFKQWKKYVG FDSWDMYNVG 60
EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA WNKLLNWYGC VEGQQPIVRK 120
VVEHGLFVKH CKVEVYLLEL KLCENSDPTN VLSCHFSKAD TIATIEKEMR KLFNIPAERE 180
TRLWNKYMSN TYEQLSKLDN TIQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SKSSTAPSRN 240
FTTSSKPSAS PYSSMSASLI ANGDSTNSSG MHNSGVSRGG AGFSASYNCQ EPPSPHIQPG 300
LCGLGNLGNT CFMNSALQCL SNTGPLTEYF LKDEYEAEIN RDNPLGMKGE IAEAYAELIK 360
QMWSGRDTHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF ILDGLHEDLN RVKKKPYLEP 420
KDANGRPDAV VAKEAWENHR LRNDSVIVDT FHGLFKSTLV CPECAKVSVT FDPFCYLTLP 480
LPLKKDRIME VFLVPADPHC RPIQYRVTVP LMGAISDLCE ALSKLSGIAA ENMVVTDVYN 540
HRFHKIFQMD EGLSHITPRD DIFVYEICTT PMDGSEYITL PVYFREKKSR PSSTSSGAVL 600
YGQPLLVSVP KHRLTLESLY QAVCERISRY IKQPLPEEFL SSPLEPGACN GSRGSYEGDE 660
EEMDHQEEGK EQLSEVEESG EDSQGGDPTE TTQKAKGPPR HKRLFTFSLV NSCGTADINS 720
LATDGKLLKL NSRSTLAIDW DSETRSLYFD EQESEACEKH TSMSQPQKKK KAAIALRECI 780
ELFTTMETLG EHDPWYCPTC KKHQQATKKF DLWSLPKILV VHLKRFSYNR YWRDKLDTVV 840
EFPVRALNMS EFVCDRAARP YVYDLIAVSN HYGAMGVGHY TAYAKNRLNG KWYYFDDSSV 900
SLASEDQIVT KAAYVLFYQR RDDECPSTSS PVSFPGSDGG AKLSSSQQDL GEEEAYTMDT 960
N 961 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0034394; P:protein localization to cell surface; ISS:UniProtKB.
 GO:0031647; P:regulation of protein stability; ISS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR006615; Pept_C19_DUSP.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF06337; DUSP
 PF00443; UCH 
SMART
 SM00695; DUSP 
PROSITE
 PS51283; DUSP
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS