CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001348
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Epsin-1 
Protein Synonyms/Alias
 EPS-15-interacting protein 1 
Gene Name
 Epn1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
23NYSEAEIKVREATSNubiquitination[1]
117QYVDRDGKDQGVNVRubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations. Regulates receptor-mediated endocytosis. 
Sequence Annotation
 DOMAIN 12 144 ENTH.
 REPEAT 183 202 UIM 1.
 REPEAT 208 227 UIM 2.
 REPEAT 233 252 UIM 3.
 REPEAT 274 276 1.
 REPEAT 294 296 2.
 REPEAT 306 308 3.
 REPEAT 319 321 4.
 REPEAT 332 334 5.
 REPEAT 349 351 6.
 REPEAT 367 369 7.
 REPEAT 377 379 8.
 REPEAT 501 503 1.
 REPEAT 517 519 2.
 REPEAT 571 573 3.
 REGION 274 379 8 X 3 AA repeats of D-P-W.
 REGION 501 573 3 X 3 AA repeats of N-P-F.
 MOTIF 401 410 [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif.
 BINDING 8 8 Phosphatidylinositol lipid headgroup.
 BINDING 11 11 Phosphatidylinositol lipid headgroup.
 BINDING 25 25 Phosphatidylinositol lipid headgroup.
 BINDING 30 30 Phosphatidylinositol lipid headgroup.
 BINDING 63 63 Phosphatidylinositol lipid headgroup.
 BINDING 73 73 Phosphatidylinositol lipid headgroup.
 MOD_RES 382 382 Phosphoserine (By similarity).
 MOD_RES 418 418 Phosphoserine (By similarity).
 MOD_RES 419 419 Phosphoserine (By similarity).
 MOD_RES 434 434 Phosphoserine (By similarity).
 MOD_RES 453 453 Phosphoserine (By similarity).
 MOD_RES 459 459 Phosphothreonine (By similarity).
 MOD_RES 469 469 Phosphothreonine (By similarity).
 MOD_RES 493 493 Phosphothreonine (By similarity).  
Keyword
 3D-structure; Cell membrane; Coated pit; Complete proteome; Cytoplasm; Endocytosis; Lipid-binding; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 575 AA 
Protein Sequence
MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV VAFSEIMSMI 60
WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM YAVQTLKDFQ YVDRDGKDQG 120
VNVREKAKQL VALLRDEDRL REERAHALKT KEKLAQTATA SSAAVGSGPP PEAEQAWPQS 180
SGEEELQLQL ALAMSKEEAD QPPSCGPEDD VQLQLALSLS REEHDKEERI RRGDDLRLQM 240
AIEESKRETG GKEESSLMDL ADVFTTPAPP QASDPWGGPA SVPTAVPVAA AASDPWGAPA 300
VPPAADPWGG AAPTPASGDP WRPAAPTGPS VDPWGGTPAP AAGEGPTSDP WGSADGGAPV 360
SGPPSSDPWA PAPAFSDPWG GSPAKPSSNG TAVGGFDTEP DEFSDFDRLR TALPTSGSST 420
GELELLAGEV PARSPGAFDM SGVGGSLAES VGSPPPAATP TPTPPTRKTP ESFLGPNAAL 480
VDLDSLVSRP GPTPPGAKAS NPFLPSGAPA TGPSVTNPFQ PAPPATLTLN QLRLSPVPPV 540
PGAPPTYISP LGGGPGLPPM MPPGPPAPNT NPFLL 575 
Gene Ontology
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0048568; P:embryonic organ development; IEA:Compara.
 GO:0006897; P:endocytosis; IDA:RGD.
 GO:0007565; P:female pregnancy; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0007219; P:Notch signaling pathway; IEA:Compara. 
Interpro
 IPR008942; ENTH_VHS.
 IPR027320; Epsin-1_mammalian.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF01417; ENTH
 PF02809; UIM 
SMART
 SM00273; ENTH
 SM00726; UIM 
PROSITE
 PS50942; ENTH
 PS50330; UIM 
PRINTS