UniProt Accession

 CENPU_HUMAN; Q71F23; A2RRD9; Q32Q71; Q9H5G1 

Genbank Protein ID

 AF469667; AF516710; AK027121; CR457376; CH471056; BC107744; BC031520; BC131556 

Genbank Nucleotide ID

 AAQ05290.1; AAQ08228.1; BAB15665.1; CAG33657.1; EAX04666.1; AAI07745.1; AAH31520.1; AAI31557.1 

Protein Name

 Centromere protein U 

Protein Synonyms/Alias

 CENP-U; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1; centromere protein of 50 kDa; CENP-50 

Gene Name

 MLF1IP 

Gene Synonyms/Alias

 CENPU; ICEN24; KLIP1; PBIP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
176	ASSELSEKPAESVTS	ubiquitination	[1]
249	GMKTSDIKELNIVLP	ubiquitination	[1]
260	IVLPEFEKTHLEHQQ	ubiquitination	[2, 3]
335	LRLEPQLKQLQTKYD	ubiquitination	[1, 4]
340	QLKQLQTKYDELKER	ubiquitination	[4]
393	SLPALLFKARTLLGA	ubiquitination	[2, 3]
413	NINHQLEKLLDQG**	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 

Sequence Annotation

 MOTIF 6 23 Nuclear localization signal (Potential).
 MOTIF 303 320 Nuclear localization signal (Potential).
 MOD_RES 78 78 Phosphothreonine; by PLK1.
 MOD_RES 110 110 Phosphothreonine.
 MOD_RES 111 111 Phosphoserine.
 MOD_RES 136 136 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 141 141 Phosphoserine.
 MOD_RES 194 194 Phosphoserine.  

Keyword

 Alternative splicing; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Host-virus interaction; Kinetochore; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 418 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HPA.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0034080; P:CENP-A containing nucleosome assembly at centromere; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR025214; NAC_CENP-U. 

Pfam

 PF13097; CENP-U 

SMART

  

PROSITE

  

PRINTS