CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002623
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-glucuronidase 
Protein Synonyms/Alias
 Beta-G1 
Gene Name
 GUSB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
39ESPSRECKELDGLWSubiquitination[1, 2, 3]
268RLLDAENKVVANGTGubiquitination[4]
359EDADIRGKGFDWPLLubiquitination[4]
368FDWPLLVKDFNLLRWubiquitination[4]
576YHLGLDQKRRKYVVGubiquitination[4]
641RYPHSVAKSQCLENSubiquitination[1, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Plays an important role in the degradation of dermatan and keratan sulfates. 
Sequence Annotation
 ACT_SITE 451 451 Proton donor.
 CARBOHYD 173 173 N-linked (GlcNAc...).
 CARBOHYD 272 272 N-linked (GlcNAc...).
 CARBOHYD 420 420 N-linked (GlcNAc...).
 CARBOHYD 631 631 N-linked (GlcNAc...).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Glycoprotein; Glycosidase; Hydrolase; Lysosome; Mucopolysaccharidosis; Polymorphism; Reference proteome; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 651 AA 
Protein Sequence
MARGSAVAWA ALGPLLWGCA LGLQGGMLYP QESPSRECKE LDGLWSFRAD FSDNRRRGFE 60
EQWYRRPLWE SGPTVDMPVP SSFNDISQDW RLRHFVGWVW YEREVILPER WTQDLRTRVV 120
LRIGSAHSYA IVWVNGVDTL EHEGGYLPFE ADISNLVQVG PLPSRLRITI AINNTLTPTT 180
LPPGTIQYLT DTSKYPKGYF VQNTYFDFFN YAGLQRSVLL YTTPTTYIDD ITVTTSVEQD 240
SGLVNYQISV KGSNLFKLEV RLLDAENKVV ANGTGTQGQL KVPGVSLWWP YLMHERPAYL 300
YSLEVQLTAQ TSLGPVSDFY TLPVGIRTVA VTKSQFLING KPFYFHGVNK HEDADIRGKG 360
FDWPLLVKDF NLLRWLGANA FRTSHYPYAE EVMQMCDRYG IVVIDECPGV GLALPQFFNN 420
VSLHHHMQVM EEVVRRDKNH PAVVMWSVAN EPASHLESAG YYLKMVIAHT KSLDPSRPVT 480
FVSNSNYAAD KGAPYVDVIC LNSYYSWYHD YGHLELIQLQ LATQFENWYK KYQKPIIQSE 540
YGAETIAGFH QDPPLMFTEE YQKSLLEQYH LGLDQKRRKY VVGELIWNFA DFMTEQSPTR 600
VLGNKKGIFT RQRQPKSAAF LLRERYWKIA NETRYPHSVA KSQCLENSLF T 651 
Gene Ontology
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0004566; F:beta-glucuronidase activity; TAS:Reactome.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0030214; P:hyaluronan catabolic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR008979; Galactose-bd-like.
 IPR006101; Glyco_hydro_2.
 IPR013812; Glyco_hydro_2/20_Ig-like.
 IPR023232; Glyco_hydro_2_AS.
 IPR023230; Glyco_hydro_2_CS.
 IPR006102; Glyco_hydro_2_Ig-like.
 IPR006104; Glyco_hydro_2_N.
 IPR006103; Glyco_hydro_2_TIM.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00703; Glyco_hydro_2
 PF02836; Glyco_hydro_2_C
 PF02837; Glyco_hydro_2_N 
SMART
  
PROSITE
 PS00719; GLYCOSYL_HYDROL_F2_1
 PS00608; GLYCOSYL_HYDROL_F2_2 
PRINTS
 PR00132; GLHYDRLASE2.