CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033343
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Probable ATP-dependent RNA helicase DHX40 
Protein Synonyms/Alias
  
Gene Name
 DHX40 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24QRVAEEMKCTLGSKVubiquitination[1]
43RFDDCSSKETAIKYMubiquitination[1, 2]
91DILFGLLKKLFQEKSubiquitination[3]
141RLYPVREKFCNLIGPubiquitination[1, 2]
319VVDGGFVKQLNHNPRubiquitination[1, 2, 4]
339LEVVPISKSEALQRSubiquitination[1, 2, 3, 5, 6]
417RLILEALKQLYQCDAubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 731 AA 
Protein Sequence
MIGVTQPRKV AAISVAQRVA EEMKCTLGSK VGYQVRFDDC SSKETAIKYM TDGCLLKHIL 60
GDPNLTKFSV IILDEAHERT LTTDILFGLL KKLFQEKSPN RKEHLKVVVM SATMELAKLS 120
AFFGNCPIFD IPGRLYPVRE KFCNLIGPRD RENTAYIQAI VKVTMDIHLN EMAGDILVFL 180
TGQFEIEKSC ELLFQMAESV DYDYDVQDTT LDGLLILPCY GSMTTVSQTS LTFVTHIQIM 240
KQISENQHSL TGLTIAGITG VSHHARPRNM TCNEKLNQQR RIFLPPPPGI RKCVISTNIS 300
ATSLTIDGIR YVVDGGFVKQ LNHNPRLGLD ILEVVPISKS EALQRSGRAG RTSSGKCFRI 360
YSKDFWNQCM PDHVIPEIKR TSLTSVVLTL KCLAIHDVIR FPYLDPPNER LILEALKQLY 420
QCDAIDRSGH VTRLGLSMVE FPLPPHLTCA VIKAASLDCE DLLLPIAAML SVENVFIRPV 480
DPEYQKEAEQ RHRELAAKAG GFNDFATLAV IFEQCKSSGA PASWCQKHWI HWRCLFSAFR 540
VEAQLRELIR KLKQQSDFPK ETFEGPKHEV LRRCLCAGYF KNVARRSVGR TFCTMDGRGS 600
PVHIHPSSAL HEQETKLEWI IFHEVLVTTK VYARIVCPIR YEWVRDLLPK LHEFNAHDLS 660
SVARREVRED ARRRWTNKEN VKQLKDGISK DVLKKMQRRN DDKSISDARA RFLERKQQRT 720
QDHSDTRKET G 731 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS