CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020031
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase DDX50 
Protein Synonyms/Alias
 DEAD box protein 50; Gu-beta; Nucleolar protein Gu2 
Gene Name
 DDX50 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87RLSDEFSKSHKSRRKubiquitination[1]
125THKSSDNKLEETLTRubiquitination[1, 2]
135ETLTREQKEGAFSNFubiquitination[1, 2]
187QARTGTGKTFSFAIPubiquitination[1]
283SGRLDLSKLRHVVLDubiquitination[1]
432SQREITLKGFREGSFubiquitination[1, 3]
506QLRYVEQKAGITFKRubiquitination[2]
524PSTMDLVKSKSMDAIubiquitination[1]
611AWKELNRKLSSNAVSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
 DOMAIN 168 347 Helicase ATP-binding.
 DOMAIN 380 524 Helicase C-terminal.
 NP_BIND 181 188 ATP (By similarity).
 MOTIF 137 165 Q motif.
 MOTIF 290 293 DEVD box.
 MOD_RES 41 41 Phosphoserine.
 MOD_RES 82 82 Phosphoserine.
 MOD_RES 86 86 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 737 AA 
Protein Sequence
MPGKLLWGDI MELEAPLEES ESQKKERQKS DRRKSRHHYD SDEKSETREN GVTDDLDAPK 60
AKKSKMKEKL NGDTEEGFNR LSDEFSKSHK SRRKDLPNGD IDEYEKKSKR VSSLDTSTHK 120
SSDNKLEETL TREQKEGAFS NFPISEETIK LLKGRGVTYL FPIQVKTFGP VYEGKDLIAQ 180
ARTGTGKTFS FAIPLIERLQ RNQETIKKSR SPKVLVLAPT RELANQVAKD FKDITRKLSV 240
ACFYGGTSYQ SQINHIRNGI DILVGTPGRI KDHLQSGRLD LSKLRHVVLD EVDQMLDLGF 300
AEQVEDIIHE SYKTDSEDNP QTLLFSATCP QWVYKVAKKY MKSRYEQVDL VGKMTQKAAT 360
TVEHLAIQCH WSQRPAVIGD VLQVYSGSEG RAIIFCETKK NVTEMAMNPH IKQNAQCLHG 420
DIAQSQREIT LKGFREGSFK VLVATNVAAR GLDIPEVDLV IQSSPPQDVE SYIHRSGRTG 480
RAGRTGICIC FYQPRERGQL RYVEQKAGIT FKRVGVPSTM DLVKSKSMDA IRSLASVSYA 540
AVDFFRPSAQ RLIEEKGAVD ALAAALAHIS GASSFEPRSL ITSDKGFVTM TLESLEEIQD 600
VSCAWKELNR KLSSNAVSQI TRMCLLKGNM GVCFDVPTTE SERLQAEWHD SDWILSVPAK 660
LPEIEEYYDG NTSSNSRQRS GWSSGRSGRS GRSGGRSGGR SGRQSRQGSR SGSRQDGRRR 720
SGNRNRSRSG GHKRSFD 737 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR012562; GUCT.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF08152; GUCT
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS