CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029760
UniProt Accession
Genbank Protein ID
 Z83840 
Genbank Nucleotide ID
  
Protein Name
 X-ray repair cross-complementing protein 6 
Protein Synonyms/Alias
  
Gene Name
 XRCC6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42VVFYGTEKDKNSVNFubiquitination[1]
44FYGTEKDKNSVNFKNubiquitination[1, 2]
50DKNSVNFKNIYVLQEubiquitination[1, 2, 3, 4, 5, 6]
64ELDNPGAKRILELDQubiquitination[1, 2, 3, 4, 6, 7, 8, 9]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 559 AA 
Protein Sequence
MKKQRKNKKR TLKQVCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK NIYVLQELDN 60
PGAKRILELD QFKGQQGQKR FQDMMGHGSD YSLSEVLWVC ANLFSDVQFK MSHKRIMLFT 120
NEDNPHGNDS AKASRARTKA GDLRDTGIFL DLMHLKKPGG FDISLFYRDI ISIAEDEDLR 180
VHFEESSKLE DLLRKVRAKE TRKRALSRLK LKLNKDIVIS VGIYNLVQKA LKPPPIKLYR 240
ETNEPVKTKT RTFNTSTGGL LLPSDTKRSQ IYGSRQIILE KEETEELKRF DDPGLMLMGF 300
KPLVLLKKHH YLRPSLFVYP EESLVIGSST LFSALLIKCL EKEVAALCRY TPRRNIPPYF 360
VALVPQEEEL DDQKIQVTPP GFQLVFLPFA DDKRKMPFTE KIMATPEQVG KMKAIVEKLR 420
FTYRSDSFEN PVLQQHFRNL EALALDLMEP EQAVDLTLPK VEAMNKRLGS LVDEFKELVY 480
PPDYNPEGKV TKRKHDNEGS GSKRPKVEYS EEELKTHISK GTLGKFTVPM LKEACRAYGL 540
KSGLKKQELL EALTKHFQD 559 
Gene Ontology
 GO:0043564; C:Ku70:Ku80 complex; IEA:InterPro.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0042162; F:telomeric DNA binding; IEA:InterPro.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
 GO:0000723; P:telomere maintenance; IEA:InterPro. 
Interpro
 IPR006165; Ku70.
 IPR006164; Ku70/Ku80_beta-barrel_dom.
 IPR027388; Ku70_bridge/pillars_dom.
 IPR005160; Ku_C.
 IPR005161; Ku_N.
 IPR003034; SAP_dom.
 IPR016194; SPOC_like_C_dom.
 IPR002035; VWF_A. 
Pfam
 PF02735; Ku
 PF03730; Ku_C
 PF03731; Ku_N
 PF02037; SAP 
SMART
 SM00559; Ku78
 SM00513; SAP 
PROSITE
 PS50800; SAP 
PRINTS