CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024761
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Activating signal cointegrator 1 complex subunit 3 
Protein Synonyms/Alias
  
Gene Name
 Ascc3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
18RSFSNVTKQDNYNEEubiquitination[1]
30NEEVADLKLKRSKLHacetylation[2]
236KYLNATLKEMTEAARubiquitination[1]
400QRDVEFEKIRYPHVYubiquitination[1]
424SAFIAGAKMILPEGIubiquitination[1]
457MPVGFEEKPVYIKDLubiquitination[1]
462EEKPVYIKDLDEVGQubiquitination[1]
473EVGQLAFKGMKRLNRubiquitination[1]
573TGDMQLSKSEILRTQacetylation[2, 3]
762PEYGHALKQVQKSRNubiquitination[1]
766HALKQVQKSRNKQVRacetylation[4]
770QVQKSRNKQVRELFSacetylation[4]
939LAYGISHKAYQIDPTubiquitination[1]
1145ILTRLEEKNLTVDKLacetylation[3]
1383AVYIAPLKALVRERMubiquitination[1]
1404IEEKLGKKVIELTGDubiquitination[1]
1544PRMASMNKPAFQAIRubiquitination[1]
2025ELQPAKTKQAWNFLSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 
Functional Description
 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3- methylcytosine (3mC) within double-stranded regions. Enhances NF- kappa-B, SRF and AP1 transactivation (By similarity). 
Sequence Annotation
 DOMAIN 487 670 Helicase ATP-binding 1.
 DOMAIN 697 915 Helicase C-terminal 1.
 DOMAIN 979 1288 SEC63 1.
 DOMAIN 1337 1512 Helicase ATP-binding 2.
 DOMAIN 1545 1740 Helicase C-terminal 2.
 DOMAIN 1813 2177 SEC63 2.
 NP_BIND 500 507 ATP (By similarity).
 NP_BIND 1350 1357 ATP (By similarity).
 MOTIF 612 615 DEVH box.
 MOTIF 1454 1457 DEIH box.
 MOD_RES 573 573 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; DNA damage; DNA repair; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2198 AA 
Protein Sequence
MALPRLTGAL RSFSNVTKQD NYNEEVADLK LKRSKLHEQV LDFGLTWKKI VKFLNEKLEK 60
NKMQNINEDL KDILQAAKQI VGTDNGREAI ESGAAFLFMT FHMTDSVGYM ETKAIRQTFG 120
PFPSSSATSA CNATNRIISH FSQDDLTAFV QMAENPCNDR VVFGKNLAFS FDMYDLDHFD 180
ELPINGESQK TISLDYKKFL NEQFQEPYTP ELKPVEKTNG SLLWCEVEKY LNATLKEMTE 240
AARVEDLCCT LYDMLASAKS GDELQDELFE LLGPEGLDLI EKLLQNRITI VDRFLNSSSD 300
HKFQVLQDSC KKILGENSKP NYGCQVTIQS EQEKQLMKQY RREEKRIARR EKKAGEDGEV 360
SGEGVLPFDP KELRIQREHA LLNARNAPIL GRQRDVEFEK IRYPHVYDSQ AQARETSAFI 420
AGAKMILPEG IQRENTKLYE EVRIPYGEPM PVGFEEKPVY IKDLDEVGQL AFKGMKRLNR 480
IQSIVFETAY NTNENMLICA PTGAGKTNIA MLTILHEIRQ HFHQGVIKKN EFKIVYVAPM 540
KALAAEMTNY FSKRLEPLGI VVKELTGDMQ LSKSEILRTQ MLVTTPEKWD VVTRKSVGDV 600
ALSQIVKLLI LDEVHLLHED RGPVLESIVA RTLRQVESTQ SMIRILGLSA TLPNYLDVAT 660
FLHVNPYIGL FYFDGRFRPV PLGQTFLGIK STNKMQQLNN MDEVCYESVL KQVKAGHQVM 720
VFVHARNATV RTAMSLIERA KNSGQISCFL PTEGPEYGHA LKQVQKSRNK QVRELFSDGF 780
SIHHAGMLRQ DRNLVENLFS NGHIKVLVCT ATLAWGVNLP AHAVVIKGTQ IYAAKRGSFV 840
DLGILDVMQI FGRAGRPQFD KFGEGIIITT HDKLSHYLSL LTQQNPIESQ FLESLADNLN 900
AEIALGTVTN VEEAVRWMSY TYLYVRMRAN PLAYGISHKA YQIDPTLRKH REQLLIEVGQ 960
KLDKAKMIRF EERTGYFSST DLGRTASHFY IKYNTIETFN ELFDAHKTEG DIFAIVSKAE 1020
EFDQIKVREE EIEELDALLN NFCELSAPGG VENSYGKINI LLQTYISRGE MDSFSLISDS 1080
AYVAQNAARI VRALFEIALR KRWPTMTYRL LNLSKVIDKR LWGWASPLRQ FSVLPPHILT 1140
RLEEKNLTVD KLKDMRKDEI GHILHHVNIG LKVKQCVHQI PSVTMEASIQ PITRTVLRVS 1200
LNIHPDFSWN DQVHGTVGEP WWIWVEDPTN DHIYHSEYFL ALKKQVINKE AQLLVFTIPI 1260
FEPLPSQYYI RAVSDRWLGA EAVCIINFQH LILPERHPPH TELLDLQPLP ITALGCKAYE 1320
ALYNFSHFNP VQTQIFHTLY HTDCNVLLGA PTGSGKTVAA ELAIFRVFNK YPTSKAVYIA 1380
PLKALVRERM DDWKIRIEEK LGKKVIELTG DVTPDMKSIA KADLIVTTPE KWDGVSRSWQ 1440
NRSYVQQVNI LIIDEIHLLG EERGPVLEVI VSRTNFISSH TEKPVRIVGL STALANARDL 1500
ADWLNIKQMG LFNFRPSVRP VPLEVHIQGF PGQHYCPRMA SMNKPAFQAI RSHSPAKPVL 1560
IFVSSRRQTR LTALELIAFL ATEEDPKQWL NMDEQEMDNI IGTVRDSNLK LTLAFGIGMH 1620
HAGLHERDRK TVEELFVNCK VQVLIATSTL AWGVNFPAHL VIIKGTEYYD GKTRRYVDFP 1680
ITDVLQMMGR AGRPQFDDQG KAVILVHDIK KDFYKKFLYE PFPVESSLLG VLSDHLNAEI 1740
AGGTITSKQD AMDYITWTYF FRRLIMNPSY YSLGDVSQDS INKFLSHLIG QSLVELELSH 1800
CIEVGEDNRT IEPLTCGRIA SYYYLKHKTV KMFKDRLKPE CSTEELLSIL SDAEEYTDLP 1860
VRHNEDHTNN ELAKCLPIEL NPHSFDSPHT KAHLLLQAHL SRAMLPCPDY DTDTKTVLDQ 1920
ALRVCQAMLD VAASQGWLVT VLNITHLIQM VIQGRWLKDS SLLTIPNIEQ HHLHLFRKWK 1980
PPVKSSHAKC RTSIECLPEL IHACEGKDHV FSSMVEKELQ PAKTKQAWNF LSRLPVINVG 2040
ISVKGSWDDL VEGHNELSIS TLTADKRDEN KWIKLHADQE YVLQVSLQRV HFGLPKGKHE 2100
NHAVTPRFPK LKDEGWFLIL GEVDKRELMA VKRVGFVRTH HDASISFFTP ETPGRYIFTL 2160
YLMSDCYLGL DQQYDIYLNV IKANISTKDS DVFTDLSV 2198 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; ISS:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008283; P:cell proliferation; ISS:UniProtKB.
 GO:0006307; P:DNA dealkylation involved in DNA repair; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR004179; Sec63-dom. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C
 PF02889; Sec63 
SMART
 SM00382; AAA
 SM00487; DEXDc
 SM00490; HELICc
 SM00611; SEC63
 SM00973; Sec63 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS