CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001847
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tryptophan--tRNA ligase 
Protein Synonyms/Alias
 Tryptophanyl-tRNA synthetase; TrpRS 
Gene Name
 trpS 
Gene Synonyms/Alias
 b3384; JW3347 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
55TVRQDAQKLRKATLDacetylation[1]
111LSRMTQFKDKSARYAacetylation[1]
113RMTQFKDKSARYAENacetylation[1]
173ALYGEIFKVPEPFIPacetylation[1]
181VPEPFIPKSGARVMSacetylation[1]
194MSLLEPTKKMSKSDDacetylation[1]
195SLLEPTKKMSKSDDNacetylation[1]
198EPTKKMSKSDDNRNNacetylation[1]
214IGLLEDPKSVVKKIKacetylation[1, 2]
218EDPKSVVKKIKRAVTacetylation[1]
268LEKQFEGKMYGHLKGacetylation[1]
274GKMYGHLKGEVADAVacetylation[1]
307AFLQQVMKDGAEKASacetylation[1]
322AHASRTLKAVYEAIGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
  
Sequence Annotation
 MOTIF 12 20 "HIGH" region.
 MOTIF 195 199 "KMSKS" region.
 BINDING 198 198 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 334 AA 
Protein Sequence
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR QDAQKLRKAT 60
LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY FGELSRMTQF KDKSARYAEN 120
INAGLFDYPV LMAADILLYQ TNLVPVGEDQ KQHLELSRDI AQRFNALYGE IFKVPEPFIP 180
KSGARVMSLL EPTKKMSKSD DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK 240
AGVSNLLDIL SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA 300
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP 334 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004830; F:tryptophan-tRNA ligase activity; IEA:HAMAP.
 GO:0006436; P:tryptophanyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002306; Trp-tRNA-ligase.
 IPR024109; Trp-tRNA-ligase_bac-type. 
Pfam
 PF00579; tRNA-synt_1b 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR01039; TRNASYNTHTRP.