CPLM-017685

Genbank Nucleotide ID

F-box only protein 22

Protein Synonyms/Alias

F-box protein FBX22p44

Homo sapiens (Human)

Position	Peptide	Type	References
40	VLTFLPAKALLRVAC	ubiquitination	[1, 2, 3, 4, 5, 6]
137	ETALALEKLFPKQCQ	ubiquitination	[5]
194	IQPFHFIKDPKNLTL	acetylation	[7]
197	FHFIKDPKNLTLERH	ubiquitination	[5]
300	NEDVSDEKTAEAAMQ	ubiquitination	[2]
337	GFQYYRAKGNVEADA	ubiquitination	[5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function.

DOMAIN 21 67 F-box.
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 194 194 N6-acetyllysine.

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Reference proteome; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005634; C:nucleus; IEA:Compara.
GO:0030018; C:Z disc; IEA:UniProtKB-SubCell.
GO:0004842; F:ubiquitin-protein ligase activity; TAS:ProtInc.
GO:0009267; P:cellular response to starvation; IEA:Compara.
GO:0006913; P:nucleocytoplasmic transport; IEA:Compara.
GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
GO:2000060; P:positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IEA:Compara.
GO:0000209; P:protein polyubiquitination; IEA:Compara.
GO:0010830; P:regulation of myotube differentiation; IEA:Compara.
GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:ProtInc.

IPR001810; F-box_dom_cyclin-like.
IPR019494; FIST_C_domain.

PF00646; F-box
PF10442; FIST_C