UniProt Accession

 TRI72_MOUSE; Q1XH17; B2RWH7; Q6NX76 

Genbank Protein ID

 AB231474; BC067209; BC147789; BC158111; BC158120 

Genbank Nucleotide ID

 BAE93014.1; AAH67209.1; AAI47790.1; AAI58112.1; AAI58121.1 

Protein Name

 Tripartite motif-containing protein 72 

Protein Synonyms/Alias

 Mitsugumin-53; Mg53 

Gene Name

 Trim72 

Gene Synonyms/Alias

 Mg53 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
137	KTQLPQQKMQLQEAC	ubiquitination	[1]
178	AVGEQLGKMRMFLAA	ubiquitination	[1]
398	LEAHVEAKEPRALRT	ubiquitination	[1]
462	VCWHDKGKNAQPLLL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. 

Sequence Annotation

 DOMAIN 271 475 B30.2/SPRY.
 ZN_FING 14 57 RING-type.
 ZN_FING 81 122 B box-type.
 DISULFID 242 242 Interchain.  

Keyword

 Cell membrane; Coiled coil; Complete proteome; Cytoplasmic vesicle; Disulfide bond; Exocytosis; Membrane; Metal-binding; Reference proteome; Transport; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 477 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
 GO:0042383; C:sarcolemma; IDA:UniProtKB.
 GO:0001786; F:phosphatidylserine binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006887; P:exocytosis; TAS:UniProtKB.
 GO:0006900; P:membrane budding; TAS:UniProtKB.
 GO:0007517; P:muscle organ development; IMP:UniProtKB.
 GO:0003012; P:muscle system process; IMP:UniProtKB.
 GO:0001778; P:plasma membrane repair; IMP:UniProtKB.
 GO:0051260; P:protein homooligomerization; IPI:UniProtKB. 

Interpro

 IPR001870; B30.2/SPRY.
 IPR003879; Butyrophylin.
 IPR008985; ConA-like_lec_gl_sf.
 IPR006574; PRY.
 IPR003877; SPRY_rcpt.
 IPR000315; Znf_B-box.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 

Pfam

 PF00622; SPRY
 PF00643; zf-B_box 

SMART

 SM00336; BBOX
 SM00589; PRY
 SM00184; RING 

PROSITE

 PS50188; B302_SPRY
 PS50119; ZF_BBOX
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS

 PR01407; BUTYPHLNCDUF.