CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000745
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein regulator of cytokinesis 1 
Protein Synonyms/Alias
  
Gene Name
 PRC1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17ESIVCLQKALNHLREubiquitination[1]
67SLKERLIKSISVCQKubiquitination[1]
273GSKAKVRKALQLEVDubiquitination[1]
602GLQRELSKASKSDATubiquitination[2]
605RELSKASKSDATSGIubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. 
Sequence Annotation
 REGION 1 341 Dimerization.
 REGION 342 466 Spectrin-fold.
 REGION 467 620 Unstructured, Arg/Lys rich.
 BINDING 377 377 Tubulin.
 BINDING 387 387 Tubulin.
 MOD_RES 195 195 Phosphoserine.
 MOD_RES 265 265 Phosphoserine.
 MOD_RES 470 470 Phosphothreonine; by CDK1.
 MOD_RES 481 481 Phosphothreonine; by CDK1.
 MOD_RES 501 501 Phosphoserine.
 MOD_RES 513 513 Phosphoserine.
 MOD_RES 571 571 Phosphoserine.
 MOD_RES 592 592 Phosphoserine.
 MOD_RES 615 615 Phosphoserine.
 MOD_RES 616 616 Phosphothreonine; by PLK1.  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 620 AA 
Protein Sequence
MRRSEVLAEE SIVCLQKALN HLREIWELIG IPEDQRLQRT EVVKKHIKEL LDMMIAEEES 60
LKERLIKSIS VCQKELNTLC SELHVEPFQE EGETTILQLE KDLRTQVELM RKQKKERKQE 120
LKLLQEQDQE LCEILCMPHY DIDSASVPSL EELNQFRQHV TTLRETKASR REEFVSIKRQ 180
IILCMEALDH TPDTSFERDV VCEDEDAFCL SLENIATLQK LLRQLEMQKS QNEAVCEGLR 240
TQIRELWDRL QIPEEEREAV ATIMSGSKAK VRKALQLEVD RLEELKMQNM KKVIEAIRVE 300
LVQYWDQCFY SQEQRQAFAP FCAEDYTESL LQLHDAEIVR LKNYYEVHKE LFEGVQKWEE 360
TWRLFLEFER KASDPNRFTN RGGNLLKEEK QRAKLQKMLP KLEEELKARI ELWEQEHSKA 420
FMVNGQKFME YVAEQWEMHR LEKERAKQER QLKNKKQTET EMLYGSAPRT PSKRRGLAPN 480
TPGKARKLNT TTMSNATANS SIRPIFGGTV YHSPVSRLPP SGSKPVAAST CSGKKTPRTG 540
RHGANKENLE LNGSILSGGY PGSAPLQRNF SINSVASTYS EFAKDPSLSD SSTVGLQREL 600
SKASKSDATS GILNSTNIQS 620 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005876; C:spindle microtubule; TAS:ProtInc.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0000910; P:cytokinesis; IDA:UniProtKB.
 GO:0000022; P:mitotic spindle elongation; TAS:ProtInc. 
Interpro
 IPR007145; MAP65_Ase1_PRC1. 
Pfam
 PF03999; MAP65_ASE1 
SMART
  
PROSITE
  
PRINTS