CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017427
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/LRR-repeat protein 6 
Protein Synonyms/Alias
 F-box and leucine-rich repeat protein 6; F-box protein FBL6; FBL6A 
Gene Name
 FBXL6 
Gene Synonyms/Alias
 FBL6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
205RLTLIHWKSQVHPVLubiquitination[1]
325LPVEALQKGCPQLQVubiquitination[1, 2]
428SDRLTLAKEGSPFLTubiquitination[1, 2, 3, 4, 5, 6]
437GSPFLTQKWCHTLREubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex (By similarity). 
Sequence Annotation
 DOMAIN 10 162 F-box.
 REPEAT 177 203 LRR 1.
 REPEAT 204 229 LRR 2.
 REPEAT 230 255 LRR 3.
 REPEAT 281 307 LRR 4.
 REPEAT 312 337 LRR 5.
 REPEAT 341 365 LRR 6.
 REPEAT 368 393 LRR 7.
 REPEAT 394 419 LRR 8.
 REPEAT 423 449 LRR 9.
 REPEAT 453 481 LRR 10.
 REPEAT 482 507 LRR 11.  
Keyword
 Alternative splicing; Complete proteome; Leucine-rich repeat; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 539 AA 
Protein Sequence
MAAPASRQVR RRARAAPRPR SAEDWWWDRL APRGSGYHLL QSDSMLLVLS EPGPARPRAQ 60
RRASRRTPRQ PPRGPSAAAK PKAGLRSEAA AAPAPAPAPT PTPEEGPDAG WGDRIPLEIL 120
VQIFGLLVAA DGPMPFLGRA ARVCRRWQEA ASQPALWHTV TLSSPLVGRP AKGGVKAEKK 180
LLASLEWLMP NRFSQLQRLT LIHWKSQVHP VLKLVGECCP RLTFLKLSGC HGVTADALVM 240
LAKACCQLHS LDLQHSMVES TAVVSFLEEA GSRMRKLWLT YSSQTTAILG ALLGSCCPQL 300
QVLEVSTGIN RNSIPLQLPV EALQKGCPQL QVLRLLNLMW LPKPPGRGVA PGPGFPSLEE 360
LCLASSTCNF VSNEVLGRLL HGSPNLRLLD LRGCARITPA GLQDLPCREL EQLHLGLYGT 420
SDRLTLAKEG SPFLTQKWCH TLRELDLSGQ GFSEKDLEQA LAAFLSTPGG SHPALCSLNL 480
RGTRVTPSTV SSVISSCPGL LYLNLESCRC LPRGLKRAYR GLEEVQWCLE QLLTSPSPS 539 
Gene Ontology
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:ProtInc.
 GO:0006508; P:proteolysis; TAS:ProtInc. 
Interpro
 IPR001810; F-box_dom_cyclin-like. 
Pfam
  
SMART
  
PROSITE
 PS50181; FBOX 
PRINTS