UniProt Accession

 ETFD_MOUSE; Q921G7; Q3U7K2; Q8BK82; Q9DCT9 

Genbank Protein ID

 AK002483; AK075673; AK141684; AK152624; AK169686; BC012522 

Genbank Nucleotide ID

 BAB22135.1; BAC35888.1; BAE24798.1; BAE31367.1; BAE41304.1; AAH12522.1 

Protein Name

 Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial 

Protein Synonyms/Alias

 ETF-QO; ETF-ubiquinone oxidoreductase; Electron-transferring-flavoprotein dehydrogenase; ETF dehydrogenase 

Gene Name

 Etfdh 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
95	QLAAEQGKDIRVCLV	acetylation	[1, 2]
124	CLDPAAFKELFPDWK	acetylation	[2]
131	KELFPDWKEKGAPLN	acetylation	[2]
133	LFPDWKEKGAPLNTP	acetylation	[2]
152	RFAILTEKHRIPVPI	acetylation	[1, 2]
222	TNDVGIQKDGAPKTT	acetylation	[1, 2]
222	TNDVGIQKDGAPKTT	ubiquitination	[3]
257	HLAKQLYKKFDLRAS	acetylation	[2]
283	ELWIIDEKKWKPGRV	acetylation	[2]
283	ELWIIDEKKWKPGRV	ubiquitination	[3]
343	FREFQRWKHHPSIQP	acetylation	[1]
356	QPTLEGGKRIAYGAR	acetylation	[2]
397	FMNVPKIKGTHTAMK	acetylation	[2]
415	LAAESIFKQLTSENL	acetylation	[2]
425	TSENLQSKTTGLHVT	ubiquitination	[3]
445	LKQSWVWKELHAVRN	acetylation	[2]
486	GMEPWTLKHKGSDSD	acetylation	[2]
496	GSDSDQLKPAKDCTP	acetylation	[2]

Reference

 [1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accepts electrons from ETF and reduces ubiquinone (By similarity). 

Sequence Annotation

 DOMAIN 576 605 4Fe-4S ferredoxin-type.
 NP_BIND 70 84 FAD (Potential).
 METAL 560 560 Iron-sulfur (4Fe-4S) (Potential).
 METAL 585 585 Iron-sulfur (4Fe-4S) (Potential).
 METAL 588 588 Iron-sulfur (4Fe-4S) (Potential).
 METAL 591 591 Iron-sulfur (4Fe-4S) (Potential).
 BINDING 304 304 Ubiquinone; via carbonyl oxygen (By
 BINDING 305 305 Ubiquinone; via amide nitrogen (By
 MOD_RES 95 95 N6-acetyllysine.
 MOD_RES 152 152 N6-acetyllysine.
 MOD_RES 222 222 N6-acetyllysine.
 MOD_RES 343 343 N6-acetyllysine.  

Keyword

 4Fe-4S; Acetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Reference proteome; Transit peptide; Transport; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 616 AA 

Protein Sequence

Gene Ontology

 GO:0031305; C:integral to mitochondrial inner membrane; IEA:Compara.
 GO:0017133; C:mitochondrial electron transfer flavoprotein complex; TAS:MGI.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; ISS:UniProtKB.
 GO:0004174; F:electron-transferring-flavoprotein dehydrogenase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0043783; F:oxidoreductase activity, oxidizing metal ions with flavin as acceptor; ISS:UniProtKB.
 GO:0048039; F:ubiquinone binding; IEA:Compara.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IDA:UniProtKB. 

Interpro

 IPR017896; 4Fe4S_Fe-S-bd.
 IPR007859; ETFD_OxRdtase.
 IPR000103; Pyridine_nuc-diS_OxRdtase_2. 

Pfam

 PF05187; ETF_QO 

SMART

  

PROSITE

 PS51379; 4FE4S_FER_2 

PRINTS

 PR00469; PNDRDTASEII.