CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044469
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 26S protease regulatory subunit 8 
Protein Synonyms/Alias
  
Gene Name
 PSMC5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MELEEGKAGSGLRQubiquitination[1, 2]
19LRQYYLSKIEELQLIubiquitination[3]
30LQLIVNDKSQNLRRLubiquitination[1, 2, 4]
47QRNELNAKVRLLREEubiquitination[1, 2, 4]
80DKKKVLVKVHPEGKFacetylation[5]
80DKKKVLVKVHPEGKFubiquitination[1, 2, 6]
86VKVHPEGKFVVDVDKubiquitination[1, 2, 3, 4, 6, 7, 8]
93KFVVDVDKNIDINDVubiquitination[1, 2, 9]
117NDSYTLHKILPNKVDubiquitination[1, 2, 3, 4, 6, 9]
122LHKILPNKVDPLVSLubiquitination[1, 2, 3, 4, 6, 9]
134VSLMMVEKVPDSTYEubiquitination[1]
148EMIGGLDKQIKEIKEubiquitination[1, 2, 3, 4]
154DKQIKEIKEVIELPVubiquitination[1, 4]
162EVIELPVKHPELFEAacetylation[10]
162EVIELPVKHPELFEAubiquitination[1, 2, 3, 4]
176ALGIAQPKGVLLYGPubiquitination[1, 4]
188YGPPGTGKTLLARAVubiquitination[1, 2, 4, 6]
214SGSELVQKFIGEGARacetylation[5]
214SGSELVQKFIGEGARubiquitination[1, 2, 3, 4, 6, 8, 9, 11]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [10] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [11] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 241 AA 
Protein Sequence
MELEEGKAGS GLRQYYLSKI EELQLIVNDK SQNLRRLQAQ RNELNAKVRL LREELQLLQE 60
QGSYVGEVVR AMDKKKVLVK VHPEGKFVVD VDKNIDINDV TPNCRVALRN DSYTLHKILP 120
NKVDPLVSLM MVEKVPDSTY EMIGGLDKQI KEIKEVIELP VKHPELFEAL GIAQPKGVLL 180
YGPPGTGKTL LARAVAHHTD CTFIRVSGSE LVQKFIGEGA RMVRELFVMA REHAPSIIFM 240
D 241 
Gene Ontology
 GO:0031595; C:nuclear proteasome complex; IEA:Compara.
 GO:0022624; C:proteasome accessory complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0016887; F:ATPase activity; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara. 
Interpro
 IPR003959; ATPase_AAA_core.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00004; AAA 
SMART
  
PROSITE
  
PRINTS