CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005107
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 cAMP-dependent protein kinase catalytic subunit beta 
Protein Synonyms/Alias
 PKA C-beta 
Gene Name
 PRKACB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73TEQYYAMKILDKQKVubiquitination[1]
77YAMKILDKQKVVKLKubiquitination[1]
286TKRFGNLKNGVSDIKubiquitination[1, 2]
318VEAPFIPKFRGSGDTubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. 
Sequence Annotation
 DOMAIN 44 298 Protein kinase.
 DOMAIN 299 351 AGC-kinase C-terminal.
 NP_BIND 50 58 ATP (By similarity).
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 73 73 ATP (By similarity).
 MOD_RES 3 3 Deamidated asparagine (By similarity).
 MOD_RES 11 11 Phosphoserine (By similarity).
 MOD_RES 49 49 Phosphothreonine (By similarity).
 MOD_RES 69 69 Phosphotyrosine (By similarity).
 MOD_RES 140 140 Phosphoserine (By similarity).
 MOD_RES 196 196 Phosphothreonine (By similarity).
 MOD_RES 198 198 Phosphothreonine (By similarity).
 MOD_RES 202 202 Phosphothreonine (By similarity).
 MOD_RES 331 331 Phosphotyrosine (By similarity).
 MOD_RES 339 339 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).  
Keyword
 Alternative splicing; ATP-binding; cAMP; Cell membrane; Complete proteome; Cytoplasm; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 351 AA 
Protein Sequence
MGNAATAKKG SEVESVKEFL AKAKEDFLKK WENPTQNNAG LEDFERKKTL GTGSFGRVML 60
VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVRLEYA FKDNSNLYMV 120
MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY 180
IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF 240
ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT 300
TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE F 351 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; TAS:UniProtKB.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004691; F:cAMP-dependent protein kinase activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0034199; P:activation of protein kinase A activity; TAS:Reactome.
 GO:0007188; P:adenylate cyclase-modulating G-protein coupled receptor signaling pathway; TAS:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0006094; P:gluconeogenesis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007243; P:intracellular protein kinase cascade; TAS:Reactome.
 GO:0051447; P:negative regulation of meiotic cell cycle; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0097338; P:response to clozapine; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0007268; P:synaptic transmission; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0019433; P:triglyceride catabolic process; TAS:Reactome.
 GO:0006833; P:water transport; TAS:Reactome. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS