UniProt Accession

 GSTA4_HUMAN; O15217; B2RD15; Q5T7Q8; Q9BX18; Q9H414 

Genbank Protein ID

 Y13047; AF020918; AF025887; AF125271; AF125272; AF125273; AF050059; AF050054; AF050055; AF050056; AF050057; AF050058; AY878121; AK315369; AL121969; AL162581; AL162581; AL121969; CH471081; BC015523 

Genbank Nucleotide ID

 CAA73482.1; AAD04711.1; AAC39695.1; AAD27704.1; AAD27705.1; AAD27706.1; AAC72706.1; AAC72706.1; AAC72706.1; AAC72706.1; AAC72706.1; AAC72706.1; AAW56075.1; BAG37762.1; CAI42451.1; CAI42451.1; CAI19517.1; CAI19517.1; EAX04394.1; AAH15523.1 

Protein Name

 Glutathione S-transferase A4 

Protein Synonyms/Alias

 GST class-alpha member 4; Glutathione S-transferase A4-4 

Gene Name

 GSTA4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
127	EVVNMAQKAIIRYFP	ubiquitination	[1, 2]
138	RYFPVFEKILRGHGQ	ubiquitination	[1, 2, 3]
203	RFLEPGSKKKPPPDE	ubiquitination	[4]
204	FLEPGSKKKPPPDEI	ubiquitination	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4- hydroxynonenal (4-HNE). 

Sequence Annotation

 DOMAIN 3 83 GST N-terminal.
 DOMAIN 85 208 GST C-terminal.
 REGION 54 55 Glutathione binding.
 REGION 67 68 Glutathione binding.
 BINDING 9 9 Glutathione.
 BINDING 212 212 Substrate.
 MOD_RES 1 1 N-acetylmethionine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 222 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004364; F:glutathione transferase activity; IDA:UniProtKB.
 GO:0006749; P:glutathione metabolic process; IDA:UniProtKB.
 GO:0006805; P:xenobiotic metabolic process; IDA:UniProtKB. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR003080; GST_alpha.
 IPR004046; GST_C.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C
 PF02798; GST_N 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS

 PR01266; GSTRNSFRASEA.