CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019209
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SAGA-associated factor 29 homolog 
Protein Synonyms/Alias
 Coiled-coil domain-containing protein 101 
Gene Name
 CCDC101 
Gene Synonyms/Alias
 SGF29 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99EERRIAAKIAGLYNDacetylation[1]
99EERRIAAKIAGLYNDubiquitination[2]
217VIPLPQWKANPETDPubiquitination[3]
288QRYVVACKEPKKK**acetylation[4]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Involved in transcriptional regulation, through association with histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. Specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3). In the SAGA- type complexes, required to recruit complexes to H3K4me. May be involved in MYC-mediated oncogenic transformation. 
Sequence Annotation
 DOMAIN 152 293 SGF29 C-terminal.
 REGION 194 196 Histone H3K4me3 N-terminus binding.
 REGION 240 243 Histone H3K4me3 N-terminus binding.
 REGION 264 266 Histone H3K4me3 binding.
 BINDING 238 238 Histone H3K4me3.
 BINDING 245 245 Histone H3K4me3.
 MOD_RES 288 288 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Chromatin regulator; Coiled coil; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 293 AA 
Protein Sequence
MALVSADSRI AELLTELHQL IKQTQEERSR SEHNLVNIQK THERMQTENK ISPYYRTKLR 60
GLYTTAKADA EAECNILRKA LDKIAEIKSL LEERRIAAKI AGLYNDSEPP RKTMRRGVLM 120
TLLQQSAMTL PLWIGKPGDK PPPLCGAIPA SGDYVARPGD KVAARVKAVD GDEQWILAEV 180
VSYSHATNKY EVDDIDEEGK ERHTLSRRRV IPLPQWKANP ETDPEALFQK EQLVLALYPQ 240
TTCFYRALIH APPQRPQDDY SVLFEDTSYA DGYSPPLNVA QRYVVACKEP KKK 293 
Gene Ontology
 GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:BHF-UCL.
 GO:0070461; C:SAGA-type complex; IDA:UniProtKB.
 GO:0035064; F:methylated histone residue binding; IDA:UniProtKB.
 GO:0071169; P:establishment of protein localization to chromatin; TAS:UniProtKB.
 GO:0043966; P:histone H3 acetylation; IDA:BHF-UCL.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR010750; SGF29_tudor-like_dom. 
Pfam
 PF07039; DUF1325 
SMART
  
PROSITE
 PS51518; SGF29_C 
PRINTS