UniProt Accession

 NSDHL_HUMAN; Q15738; D3DWT6; O00344 

Genbank Protein ID

 U47105; U82671; CH471172; CH471172; BC000245; BC007816 

Genbank Nucleotide ID

 AAC50558.2; EAW72898.1; EAW72899.1; AAH00245.1; AAH07816.1 

Protein Name

 Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating 

Protein Synonyms/Alias

 Protein H105e3 

Gene Name

 NSDHL 

Gene Synonyms/Alias

 H105E3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
31	KVNADIEKVNQNQAK	ubiquitination	[1, 2]
96	QDLYPALKGVNTVFH	ubiquitination	[3, 4, 5]
191	LGANDPEKNFLTTAI	ubiquitination	[3, 5, 6]
263	RDSTLGGKAFHITND	ubiquitination	[7]
345	YSCERAKKAMGYQPL	ubiquitination	[6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

  

Sequence Annotation

 ACT_SITE 172 172 Proton acceptor (By similarity).
 BINDING 176 176 NAD (By similarity).  

Keyword

 Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Disease mutation; Ichthyosis; Lipid biosynthesis; Lipid metabolism; Membrane; Mental retardation; NAD; Oxidoreductase; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 373 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005811; C:lipid particle; IDA:UniProtKB.
 GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; TAS:ProtInc.
 GO:0047012; F:sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; IEA:EC.
 GO:0006695; P:cholesterol biosynthetic process; TAS:Reactome.
 GO:0001942; P:hair follicle development; IEA:Compara.
 GO:0060716; P:labyrinthine layer blood vessel development; IEA:Compara.
 GO:0007224; P:smoothened signaling pathway; IEA:Compara. 

Interpro

 IPR002225; 3Beta_OHSteriod_DH/Estase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF01073; 3Beta_HSD 

SMART

  

PROSITE

  

PRINTS