CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004515
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 12 
Protein Synonyms/Alias
 Gonadotropin-inducible ovary transcription repressor 3; GIOT-3; Zinc finger protein 325; Zinc finger protein KOX3 
Gene Name
 ZNF12 
Gene Synonyms/Alias
 GIOT3; KOX3; ZNF325 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
209NEESLYQKIRILEKPubiquitination[1]
215QKIRILEKPFEYIECubiquitination[1]
337CGKNFYQKLHLIQHQubiquitination[1, 2]
393CGKTFSQKSALNDHQubiquitination[1]
491QRVHTGEKPYECNECubiquitination[1]
575HRIHSGEKPYECSECubiquitination[3]
603QRTHTGEKAYECYECubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcriptional repressor which suppresses activation protein 1 (AP-1)- and serum response element (SRE)-mediated transcriptional activity. 
Sequence Annotation
 DOMAIN 8 79 KRAB.
 ZN_FING 269 291 C2H2-type 1.
 ZN_FING 297 319 C2H2-type 2.
 ZN_FING 325 347 C2H2-type 3.
 ZN_FING 353 375 C2H2-type 4.
 ZN_FING 381 403 C2H2-type 5.
 ZN_FING 409 431 C2H2-type 6.
 ZN_FING 437 459 C2H2-type 7.
 ZN_FING 465 487 C2H2-type 8.
 ZN_FING 493 515 C2H2-type 9.
 ZN_FING 521 543 C2H2-type 10.
 ZN_FING 549 571 C2H2-type 11.
 ZN_FING 577 599 C2H2-type 12.
 ZN_FING 605 627 C2H2-type 13.
 ZN_FING 633 655 C2H2-type 14.
 ZN_FING 661 683 C2H2-type 15.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 697 AA 
Protein Sequence
MNKSLGPVSF KDVAVDFTQE EWQQLDPEQK ITYRDVMLEN YSNLVSVGYH IIKPDVISKL 60
EQGEEPWIVE GEFLLQSYPD EVWQTDDLIE RIQEEENKPS RQTVFIETLI EERGNVPGKT 120
FDVETNPVPS RKIAYKNSLC DSCEKCLTSV SEYISSDGSY ARMKADECSG CGKSLLHIKL 180
EKTHPGDQAY EFNQNGEPYT LNEESLYQKI RILEKPFEYI ECQKAFQKDT VFVNHMEEKP 240
YKWNGSEIAF LQMSDLTVHQ TSHMEMKPYE CSECGKSFCK KSKFIIHQRT HTGEKPYECN 300
QCGKSFCQKG TLTVHQRTHT GEKPYECNEC GKNFYQKLHL IQHQRTHSGE KPYECSYCGK 360
SFCQKTHLTQ HQRTHSGERP YVCHDCGKTF SQKSALNDHQ KIHTGVKLYK CSECGKCFCR 420
KSTLTTHLRT HTGEKPYECN ECGKFFSRLS YLTVHYRTHS GEKPYECNEC GKTFYLNSAL 480
MRHQRVHTGE KPYECNECGK LFSQLSYLTI HHRTHSGVKP YECSECGKTF YQNSALCRHR 540
RIHKGEKPYE CYICGKFFSQ MSYLTIHHRI HSGEKPYECS ECGKTFCQNS ALNRHQRTHT 600
GEKAYECYEC GKCFSQMSYL TIHHRIHSGE KPFECNECGK AFSRMSYLTV HYRTHSGEKP 660
YECTECGKKF YHKSAFNSHQ RIHRRGNMNV IDVGRLL 697 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS