UniProt Accession

 2AAA_MOUSE; Q76MZ3 

Genbank Protein ID

 AB021743; AK054239; AK078135; BC006606 

Genbank Nucleotide ID

 BAA75478.1; BAC35700.1; BAC37143.1; AAH06606.1 

Protein Name

 Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform 

Protein Synonyms/Alias

 PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha 

Gene Name

 Ppp2r1a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
34	LRLNSIKKLSTIALA	ubiquitination	[1]
107	EETVVRDKAVESLRA	ubiquitination	[1]
163	PRVSSAVKAELRQYF	acetylation	[2]
163	PRVSSAVKAELRQYF	ubiquitination	[1]
188	VRRAAASKLGEFAKV	ubiquitination	[1]
194	SKLGEFAKVLELDNV	acetylation	[2]
266	VRYMVADKFTELQKA	ubiquitination	[1]
272	DKFTELQKAVGPEIT	acetylation	[2]
272	DKFTELQKAVGPEIT	ubiquitination	[1]
280	AVGPEITKTDLVPAF	acetylation	[2, 3, 4, 5]
280	AVGPEITKTDLVPAF	ubiquitination	[1]
307	AAASHKVKEFCENLS	ubiquitination	[1]
546	NVAKSLQKIGPILDN	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis (By similarity). 

Sequence Annotation

 REPEAT 8 46 HEAT 1.
 REPEAT 47 84 HEAT 2.
 REPEAT 85 123 HEAT 3.
 REPEAT 124 161 HEAT 4.
 REPEAT 162 200 HEAT 5.
 REPEAT 201 239 HEAT 6.
 REPEAT 240 278 HEAT 7.
 REPEAT 279 321 HEAT 8.
 REPEAT 322 360 HEAT 9.
 REPEAT 361 399 HEAT 10.
 REPEAT 400 438 HEAT 11.
 REPEAT 439 477 HEAT 12.
 REPEAT 478 516 HEAT 13.
 REPEAT 517 555 HEAT 14.
 REPEAT 556 589 HEAT 15.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 280 280 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Centromere; Chromosome; Chromosome partition; Complete proteome; Cytoplasm; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 589 AA 

Protein Sequence

Gene Ontology

 GO:0000775; C:chromosome, centromeric region; ISS:UniProtKB.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0000159; C:protein phosphatase type 2A complex; IDA:MGI.
 GO:0003823; F:antigen binding; IEA:Compara.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:MGI.
 GO:0007059; P:chromosome segregation; ISS:UniProtKB.
 GO:0070262; P:peptidyl-serine dephosphorylation; IDA:MGI.
 GO:2001241; P:positive regulation of extrinsic apoptotic signaling pathway in absence of ligand; IMP:MGI. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000357; HEAT.
 IPR021133; HEAT_type_2. 

Pfam

 PF02985; HEAT 

SMART

  

PROSITE

 PS50077; HEAT_REPEAT 

PRINTS