UniProt Accession

 CHKA_HUMAN; P35790; Q8NE29 

Genbank Protein ID

 D10704; AP002807; AP002992; BC036471 

Genbank Nucleotide ID

 BAA01547.1; AAH36471.1 

Protein Name

 Choline kinase alpha 

Protein Synonyms/Alias

 CK; CHETK-alpha; Ethanolamine kinase; EK 

Gene Name

 CHKA 

Gene Synonyms/Alias

 CHK; CKI 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
96	RRAYLWCKEFLPGAW	ubiquitination	[1]
195	AERSLGPKLYGIFPQ	ubiquitination	[2, 3]
258	GTMEKYLKEVLRIKF	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. 

Sequence Annotation

 NP_BIND 117 123 ATP.
 NP_BIND 207 213 ATP.
 REGION 119 121 Substrate binding.
 BINDING 146 146 ATP.
 BINDING 308 308 ATP (Probable).
 BINDING 330 330 ATP.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Lipid biosynthesis; Lipid metabolism; Nucleotide-binding; Phospholipid biosynthesis; Phospholipid metabolism; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 457 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0033265; F:choline binding; IEA:Compara.
 GO:0004103; F:choline kinase activity; IDA:UniProtKB.
 GO:0004104; F:cholinesterase activity; IEA:Compara.
 GO:0008144; F:drug binding; IDA:UniProtKB.
 GO:0004305; F:ethanolamine kinase activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0019695; P:choline metabolic process; IEA:Compara.
 GO:0006869; P:lipid transport; TAS:ProtInc.
 GO:0006646; P:phosphatidylethanolamine biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR026712; Cho/Etha_kinase.
 IPR011009; Kinase-like_dom. 

Pfam

  

SMART

  

PROSITE

  

PRINTS