| Tag | Content |
|---|
| CPLM ID | CPLM-021894 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Pyruvate carboxylase |
Protein Synonyms/Alias | Pyruvic carboxylase; PYC |
Gene Name | pyc |
Gene Synonyms/Alias | pycA; ylaP; BSU14860 |
Created Date | July 27, 2013 |
Organism | Bacillus subtilis (strain 168) |
NCBI Taxa ID | 224308 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 328 | QGHSLHSKKVNIPEQ | acetylation | [1] | | 719 | KDMAGLLKPQAAYEL | acetylation | [1] |
|
Reference | [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis. Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG. Proteomics. 2013 May;13(10-11):1726-36. [ PMID: 23468065] |
Functional Description | Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second, leading to oxaloacetate production. Fulfills an anaplerotic function in B.subtilis as it is necessary for growth on glucose, but is not required for sporulation. |
Sequence Annotation | DOMAIN 1 457 Biotin carboxylation.
DOMAIN 125 321 ATP-grasp.
DOMAIN 534 802 Carboxyltransferase.
DOMAIN 1078 1148 Biotinyl-binding.
REGION 542 546 Substrate binding (By similarity).
ACT_SITE 242 242 Potential.
METAL 543 543 Divalent metal cation (By similarity).
METAL 712 712 Divalent metal cation; via carbamate
METAL 741 741 Divalent metal cation (By similarity).
METAL 743 743 Divalent metal cation (By similarity).
BINDING 121 121 ATP (By similarity).
BINDING 205 205 ATP (By similarity).
BINDING 240 240 ATP (By similarity).
BINDING 615 615 Substrate (By similarity).
BINDING 876 876 Substrate (By similarity).
MOD_RES 712 712 N6-carboxylysine (By similarity).
MOD_RES 1112 1112 N6-biotinyllysine (By similarity). |
Keyword | ATP-binding; Biotin; Complete proteome; Ligase; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1148 AA |
Protein Sequence | MSQQSIQKVL VANRGEIAIR IFRACTELNI RTVAVYSKED SGSYHRYKAD EAYLVGEGKK 60
PIDAYLDIEG IIDIAKRNKV DAIHPGYGFL SENIHFARRC EEEGIVFIGP KSEHLDMFGD 120
KVKAREQAEK AGIPVIPGSD GPAETLEAVE QFGQANGYPI IIKASLGGGG RGMRIVRSES 180
EVKEAYERAK SEAKAAFGND EVYVEKLIEN PKHIEVQVIG DKQGNVVHLF ERDCSVQRRH 240
QKVIEVAPSV SLSPELRDQI CEAAVALAKN VNYINAGTVE FLVANNEFYF IEVNPRVQVE 300
HTITEMITGV DIVQTQILVA QGHSLHSKKV NIPEQKDIFT IGYAIQSRVT TEDPQNDFMP 360
DTGKIMAYRS GGGFGVRLDT GNSFQGAVIT PYYDSLLVKL STWALTFEQA AAKMVRNLQE 420
FRIRGIKTNI PFLENVAKHE KFLTGQYDTS FIDTTPELFN FPKQKDRGTK MLTYIGNVTV 480
NGFPGIGKKE KPAFDKPLGV KVDVDQQPAR GTKQILDEKG AEGLANWVKE QKSVLLTDTT 540
FRDAHQSLLA TRIRSHDLKK IANPTAALWP ELFSMEMWGG ATFDVAYRFL KEDPWKRLED 600
LRKEVPNTLF QMLLRSSNAV GYTNYPDNVI KEFVKQSAQS GIDVFRIFDS LNWVKGMTLA 660
IDAVRDTGKV AEAAICYTGD ILDKNRTKYD LAYYTSMAKE LEAAGAHILG IKDMAGLLKP 720
QAAYELVSAL KETIDIPVHL HTHDTSGNGI YMYAKAVEAG VDIIDVAVSS MAGLTSQPSA 780
SGFYHAMEGN DRRPEMNVQG VELLSQYWES VRKYYSEFES GMKSPHTEIY EHEMPGGQYS 840
NLQQQAKGVG LGDRWNEVKE MYRRVNDMFG DIVKVTPSSK VVGDMALYMV QNNLTEKDVY 900
EKGESLDFPD SVVELFKGNI GQPHGGFPEK LQKLILKGQE PITVRPGELL EPVSFEAIKQ 960
EFKEQHNLEI SDQDAVAYAL YPKVFTDYVK TTESYGDISV LDTPTFFYGM TLGEEIEVEI 1020
ERGKTLIVKL ISIGEPQPDA TRVVYFELNG QPREVVIKDE SIKSSVQERL KADRTNPSHI 1080
AASMPGTVIK VLAEAGTKVN KGDHLMINEA MKMETTVQAP FSGTIKQVHV KNGEPIQTGD 1140
LLLEIEKA 1148 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |