CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006853
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peroxisomal-coenzyme A synthetase 
Protein Synonyms/Alias
  
Gene Name
 PCS60 
Gene Synonyms/Alias
 FAT2; YBR222C; YBR1512 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
173YRSLNNAKFVNTNPVacetylation[1]
434LVLTGRIKELINRGGubiquitination[2]
515TKVYFVDKLPKTATGacetylation[1]
523LPKTATGKIQRRVIAubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Non-essential protein involved in fatty acid metabolism. 
Sequence Annotation
 NP_BIND 190 207 AMP (By similarity).
 MOTIF 541 543 Microbody targeting signal (Potential).  
Keyword
 Complete proteome; Direct protein sequencing; Ligase; Membrane; Peroxisome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 543 AA 
Protein Sequence
MTSAATVTAS FNDTFSVSDN VAVIVPETDT QVTYRDLSHM VGHFQTMFTN PNSPLYGAVF 60
RQDTVAISMR NGLEFIVAFL GATMDAKIGA PLNPNYKEKE FNFYLNDLKS KAICVPKGTT 120
KLQSSEILKS ASTFGCFIVE LAFDATRFRV EYDIYSPEDN YKRVIYRSLN NAKFVNTNPV 180
KFPGFARSSD VALILHTSGT TSTPKTVPLL HLNIVRSTLN IANTYKLTPL DRSYVVMPLF 240
HVHGLIGVLL STFRTQGSVV VPDGFHPKLF WDQFVKYNCN WFSCVPTISM IMLNMPKPNP 300
FPHIRFIRSC SSALAPATFH KLEKEFNAPV LEAYAMTEAS HQMTSNNLPP GKRKPGTVGQ 360
PQGVTVVILD DNDNVLPPGK VGEVSIRGEN VTLGYANNPK ANKENFTKRE NYFRTGDQGY 420
FDPEGFLVLT GRIKELINRG GEKISPIELD GIMLSHPKID EAVAFGVPDD MYGQVVQAAI 480
VLKKGEKMTY EELVNFLKKH LASFKIPTKV YFVDKLPKTA TGKIQRRVIA ETFAKSSRNK 540
SKL 543 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005782; C:peroxisomal matrix; IDA:SGD.
 GO:0005778; C:peroxisomal membrane; IDA:SGD.
 GO:0016208; F:AMP binding; ISS:SGD.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0003729; F:mRNA binding; IDA:SGD. 
Interpro
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig.
 IPR025110; DUF4009. 
Pfam
 PF00501; AMP-binding
 PF13193; DUF4009 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS