CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012454
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MAD2L1-binding protein 
Protein Synonyms/Alias
 Caught by MAD2 protein 
Gene Name
 MAD2L1BP 
Gene Synonyms/Alias
 CMT2; KIAA0110 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
95PLPYEQLKHFYRKPSubiquitination[1, 2, 3, 4, 5]
100QLKHFYRKPSPQAEEubiquitination[2, 4]
110PQAEEMLKKKPRATTubiquitination[2, 4, 6]
229GEDWFRPKLNYRVPSubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. 
Sequence Annotation
 REGION 45 78 Interaction with MAD2L1.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 274 AA 
Protein Sequence
MAAPEAEVLS SAAVPDLEWY EKSEETHASQ IELLETSSTQ EPLNASEAFC PRDCMVPVVF 60
PGPVSQEGCC QFTCELLKHI MYQRQQLPLP YEQLKHFYRK PSPQAEEMLK KKPRATTEVS 120
SRKCQQALAE LESVLSHLED FFARTLVPRV LILLGGNALS PKEFYELDLS LLAPYSVDQS 180
LSTAACLRRL FRAIFMADAF SELQAPPLMG TVVMAQGHRN CGEDWFRPKL NYRVPSRGHK 240
LTVTLSCGRP SIRTTAWEDY IWFQAPVTFK GFRE 274 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0007093; P:mitotic cell cycle checkpoint; IMP:UniProtKB.
 GO:0007096; P:regulation of exit from mitosis; IMP:UniProtKB. 
Interpro
 IPR009511; MAD1/Cdc20-bound-Mad2-bd. 
Pfam
 PF06581; p31comet 
SMART
  
PROSITE
  
PRINTS