UniProt Accession

 POB3_YEAST; Q04636; D6VZA4 

Genbank Protein ID

 Z38114; BK006946 

Genbank Nucleotide ID

 CAA86251.1; DAA09828.1 

Protein Name

 FACT complex subunit POB3 

Protein Synonyms/Alias

 Facilitates chromatin transcription complex subunit POB3 

Gene Name

 POB3 

Gene Synonyms/Alias

 YML069W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
191	VDENMTKKEESSNEV	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication. 

Sequence Annotation

  

Keyword

 3D-structure; Chromosome; Complete proteome; DNA damage; DNA repair; DNA replication; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 552 AA 

Protein Sequence

Gene Ontology

 GO:0035101; C:FACT complex; IDA:SGD.
 GO:0000790; C:nuclear chromatin; IDA:SGD.
 GO:0031298; C:replication fork protection complex; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0034724; P:DNA replication-independent nucleosome organization; IDA:SGD.
 GO:0006261; P:DNA-dependent DNA replication; IMP:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IDA:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IC:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR013719; DUF1747.
 IPR011993; PH_like_dom.
 IPR000969; SSrcognition.
 IPR024954; SSRP1_dom. 

Pfam

 PF08512; Rtt106
 PF03531; SSrecog 

SMART

  

PROSITE

  

PRINTS

 PR00887; SSRCOGNITION.