CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022369
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein AATF 
Protein Synonyms/Alias
 Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1 
Gene Name
 AATF 
Gene Synonyms/Alias
 CHE1; DED; HSPC277 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
69ASLLDTDKRYCGKTTubiquitination[1]
159QSISDFEKFTKGMDDubiquitination[1, 2, 3, 4, 5, 6]
228VEKGRAVKNQIALWDubiquitination[1, 3, 4, 7]
243QLLEGRIKLQKALLTubiquitination[4]
246EGRIKLQKALLTTNQubiquitination[1, 2, 3, 4, 5, 7]
264PDVFPLFKDKGGPEFubiquitination[1, 3, 7]
266VFPLFKDKGGPEFSSubiquitination[1, 4, 6, 7]
280SALKNSHKALKALLRubiquitination[4]
342RRVPAKRKLEMEDYPubiquitination[1, 4]
354DYPSFMAKRFADFTVubiquitination[1, 3, 4, 7]
383LASGKLGKGFGAFERubiquitination[1, 4, 8]
403IDHILMDKERLLRRTubiquitination[1, 2, 4, 5]
422SVYRVLGKPEPAAQPubiquitination[1]
475LRELIERKTSSLDPNubiquitination[1, 4, 7, 9]
496RQWLAIQKLRSKIHKubiquitination[1, 2, 4, 5, 10]
522LRFHVLSKLLSFMAPubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. 
Sequence Annotation
 REGION 273 315 POLR2J binding.
 REGION 316 372 RB1 binding.
 REGION 373 472 RB1 and SP1 binding.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 316 316 Phosphoserine.
 MOD_RES 320 320 Phosphoserine.
 MOD_RES 321 321 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 560 AA 
Protein Sequence
MAGPQPLALQ LEQLLNPRPS EADPEADPEE ATAARVIDRF DEGEDGEGDF LVVGSIRKLA 60
SASLLDTDKR YCGKTTSRKA WNEDHWEQTL PGSSDEEISD EEGSGDEDSE GLGLEEYDED 120
DLGAAEEQEC GDHRESKKSR SHSAKTPGFS VQSISDFEKF TKGMDDLGSS EEEEDEESGM 180
EEGDDAEDSQ GESEEDRAGD RNSEDDGVVM TFSSVKVSEE VEKGRAVKNQ IALWDQLLEG 240
RIKLQKALLT TNQLPQPDVF PLFKDKGGPE FSSALKNSHK ALKALLRSLV GLQEELLFQY 300
PDTRYLVDGT KPNAGSEEIS SEDDELVEEK KQQRRRVPAK RKLEMEDYPS FMAKRFADFT 360
VYRNRTLQKW HDKTKLASGK LGKGFGAFER SILTQIDHIL MDKERLLRRT QTKRSVYRVL 420
GKPEPAAQPV PESLPGEPEI LPQAPANAHL KDLDEEIFDD DDFYHQLLRE LIERKTSSLD 480
PNDQVAMGRQ WLAIQKLRSK IHKKVDRKAS KGRKLRFHVL SKLLSFMAPI DHTTMNDDAR 540
TELYRSLFGQ LHPPDEGHGD 560 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0007155; P:cell adhesion; IEA:Compara.
 GO:0040016; P:embryonic cleavage; IEA:Compara.
 GO:0042985; P:negative regulation of amyloid precursor protein biosynthetic process; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0032929; P:negative regulation of superoxide anion generation; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0007346; P:regulation of mitotic cell cycle; IEA:Compara.
 GO:0006974; P:response to DNA damage stimulus; IEP:UniProtKB.
 GO:0042254; P:ribosome biogenesis; IEA:Compara. 
Interpro
 IPR025160; AATF.
 IPR012617; AATF_C. 
Pfam
 PF13339; AATF-Che1
 PF08164; TRAUB 
SMART
  
PROSITE
  
PRINTS