UniProt Accession

 SPSY_HUMAN; P52788; A6NHA7; A6NI34; B2R9M0; O00544; Q9UQS1 

Genbank Protein ID

 Z49099; AD001528; AK313834; U53331; U73023; CH471074; BC009898; BC085621 

Genbank Nucleotide ID

 CAA88921.1; AAB61308.1; BAG36567.1; AAD08634.1; EAW98984.1; AAH09898.1; AAH85621.1 

Protein Name

 Spermine synthase 

Protein Synonyms/Alias

 SPMSY; Spermidine aminopropyltransferase 

Gene Name

 SMS 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
16	LDFMLGAKADGETIL	ubiquitination	[1]
98	NKVEERMKELSQDST	ubiquitination	[2]
151	DSPYQNIKILHSKQF	ubiquitination	[2, 3, 4, 5]
186	RAIMGSGKEDYTGKD	ubiquitination	[4]
230	QMVIDGCKKYMRKTC	ubiquitination	[4]
235	GCKKYMRKTCGDVLD	ubiquitination	[6]

Reference

 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM). 

Sequence Annotation

 REGION 255 256 S-adenosylmethioninamine binding.
 ACT_SITE 276 276 Proton acceptor (Probable).
 BINDING 148 148 S-adenosylmethioninamine.
 BINDING 177 177 Spermidine.
 BINDING 201 201 Spermidine.
 BINDING 220 220 S-adenosylmethioninamine.
 BINDING 351 351 Spermidine.
 BINDING 353 353 Spermidine.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Mental retardation; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 366 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004766; F:spermidine synthase activity; TAS:ProtInc.
 GO:0016768; F:spermine synthase activity; IEA:EC.
 GO:0006555; P:methionine metabolic process; TAS:ProtInc.
 GO:0006595; P:polyamine metabolic process; TAS:Reactome.
 GO:0006597; P:spermine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001045; Spermidine/spermine_synthase.
 IPR015576; Spermine_synthase. 

Pfam

 PF01564; Spermine_synth 

SMART

  

PROSITE

 PS01330; SPERMIDINE_SYNTHASE_1
 PS51006; SPERMIDINE_SYNTHASE_2 

PRINTS