CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007257
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit beta-4 
Protein Synonyms/Alias
 Importin-123; Karyopherin subunit beta-4; Karyopherin-123; Ran-binding protein YRB4 
Gene Name
 KAP123 
Gene Synonyms/Alias
 YRB4; YER110C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
69EARKLVSKHWNAIDEacetylation[1]
95QTAFSEPKENVRHSNacetylation[1]
118TEELDGNKWPDLVPNubiquitination[2]
296KSKVSQSKLGPEITVacetylation[1]
407PATINGLKDTEPIVKacetylation[1]
614ENFAPFLKTILPEIFubiquitination[3]
725KSVLLASKVEPESYPacetylation[1]
725KSVLLASKVEPESYPubiquitination[3]
916VIRLTSDKSLEVRGNacetylation[1]
1086QTEEMKHKVIELLKYacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for nuclear protein import, its predominant substrate seems to be ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran). 
Sequence Annotation
 DOMAIN 25 92 Importin N-terminal.
 REPEAT 117 154 HEAT 1.
 REPEAT 158 196 HEAT 2.
 REPEAT 393 430 HEAT 3.
 REPEAT 434 472 HEAT 4.
 REPEAT 477 515 HEAT 5.
 REPEAT 522 565 HEAT 6.
 REPEAT 570 607 HEAT 7.
 REPEAT 611 649 HEAT 8.
 REPEAT 859 896 HEAT 9.
 REPEAT 941 979 HEAT 10.
 REPEAT 1016 1053 HEAT 11.
 MOD_RES 646 646 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1113 AA 
Protein Sequence
MDQQFLSQLE QTLHAITSGV GLKEATKTLQ TQFYTQPTTL PALIHILQNG SDDSLKQLAG 60
VEARKLVSKH WNAIDESTRA SIKTSLLQTA FSEPKENVRH SNARVIASIG TEELDGNKWP 120
DLVPNLIQTA SGEDVQTRQT AIFILFSLLE DFTSSLSGHI DDFLALFSQT INDPSSLEIR 180
SLSAQALNHV SALIEEQETI NPVQAQKFAA SIPSVVNVLD AVIKADDTMN AKLIFNCLND 240
FLLLDSQLTG NFIVDLIKLS LQIAVNSEID EDVRVFALQF IISSLSYRKS KVSQSKLGPE 300
ITVAALKVAC EEIDVDDELN NEDETGENEE NTPSSSAIRL LAFASSELPP SQVASVIVEH 360
IPAMLQSANV FERRAILLAI SVAVTGSPDY ILSQFDKIIP ATINGLKDTE PIVKLAALKC 420
IHQLTTDLQD EVAKFHEEYL PLIIDIIDSA KNIVIYNYAT VALDGLLEFI AYDAIAKYLD 480
PLMNKLFYML ESNESSKLRC AVVSAIGSAA FAAGSAFIPY FKTSVHYLEK FIQNCSQIEG 540
MSEDDIELRA NTFENISTMA RAVRSDAFAE FAEPLVNSAY EAIKTDSARL RESGYAFIAN 600
LAKVYGENFA PFLKTILPEI FKTLELDEYQ FNFDGDAEDL AAFADSANEE ELQNKFTVNT 660
GISYEKEVAS AALSELALGT KEHFLPYVEQ SLKVLNEQVD ESYGLRETAL NTIWNVVKSV 720
LLASKVEPES YPKGIPASSY VNADVLAVIQ AARETSMGNL SDEFETSMVI TVMEDFANMI 780
KQFGAIIIMD NGDSSMLEAL CMQVLSVLKG THTCQTIDIE EDVPRDEELD ASETEATLQD 840
VALEVLVSLS QALAGDFAKV FDNFRPVVFG LFQSKSKNKR SSAVGAASEL ALGMKEQNPF 900
VHEMLEALVI RLTSDKSLEV RGNAAYGVGL LCEYASMDIS AVYEPVLKAL YELLSAADQK 960
ALAAEDDEAT REIIDRAYAN ASGCVARMAL KNSALVPLEQ TVPALLAHLP LNTGFEEYNP 1020
IFELIMKLYQ ENSPVITNET PRIIEIFSAV FTKENDRIKL EKESTLGREE NMERLKQFQT 1080
EEMKHKVIEL LKYLNTTYNG IVAQNPVLAA VIA 1113 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005643; C:nuclear pore; IDA:SGD.
 GO:0008139; F:nuclear localization sequence binding; IDA:SGD.
 GO:0008565; F:protein transporter activity; IMP:SGD.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0006607; P:NLS-bearing substrate import into nucleus; IMP:SGD.
 GO:2000220; P:regulation of pseudohyphal growth; IMP:SGD.
 GO:0006610; P:ribosomal protein import into nucleus; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021133; HEAT_type_2.
 IPR001494; Importin-beta_N. 
Pfam
 PF03810; IBN_N 
SMART
 SM00913; IBN_N 
PROSITE
 PS50077; HEAT_REPEAT
 PS50166; IMPORTIN_B_NT 
PRINTS