UniProt Accession

 CTNA1_MOUSE; P26231 

Genbank Protein ID

 X59990; D90362; BC048163 

Genbank Nucleotide ID

 CAA42607.1; BAA14376.1; AAH48163.1 

Protein Name

 Catenin alpha-1 

Protein Synonyms/Alias

 102 kDa cadherin-associated protein; Alpha E-catenin; CAP102 

Gene Name

 Ctnna1 

Gene Synonyms/Alias

 Catna1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
12	HAGNINFKWDPKSLE	ubiquitination	[1]
16	INFKWDPKSLEIRTL	ubiquitination	[1]
45	TLVNTNSKGPSNKKR	ubiquitination	[1]
57	KKRGRSKKAHVLAAS	ubiquitination	[1]
75	ATENFLEKGDKIAKE	ubiquitination	[1]
81	EKGDKIAKESQFLKE	ubiquitination	[1]
163	VVEDGILKLRNAGNE	ubiquitination	[1]
178	QDLGIQYKALKPEVD	ubiquitination	[1]
181	GIQYKALKPEVDKLN	ubiquitination	[1]
186	ALKPEVDKLNIMAAK	acetylation	[2, 3, 4]
193	KLNIMAAKRQQELKD	acetylation	[3]
193	KLNIMAAKRQQELKD	ubiquitination	[1]
216	AARGILQKNVPILYT	ubiquitination	[1]
417	NGNEKEVKEYAQVFR	ubiquitination	[1]
478	LAAKPQSKLAQENMD	ubiquitination	[1]
571	EPGVYTEKVLEATKL	ubiquitination	[1]
577	EKVLEATKLLSNTVM	ubiquitination	[1]
681	AQLPQEQKAKIAEQV	ubiquitination	[1]
683	LPQEQKAKIAEQVAS	ubiquitination	[1]
695	VASFQEEKSKLDAEV	acetylation	[3]
695	VASFQEEKSKLDAEV	ubiquitination	[1]
697	SFQEEKSKLDAEVSK	ubiquitination	[1]
704	KLDAEVSKWDDSGND	ubiquitination	[1]
737	TRGKGPLKNTSDVIS	ubiquitination	[1]
797	LNICSKVKAEVQNLG	ubiquitination	[1]
842	ASYVASTKYQKSQGM	ubiquitination	[1]
845	VASTKYQKSQGMASL	ubiquitination	[1]
860	NLPAVSWKMKAPEKK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. 

Sequence Annotation

 REGION 2 228 Involved in homodimerization (By
 REGION 97 148 Interaction with JUP and CTNNB1 (By
 REGION 325 394 Interaction with alpha-actinin (By
 MOD_RES 2 2 N-acetylthreonine (By similarity).
 MOD_RES 634 634 Phosphothreonine (By similarity).
 MOD_RES 641 641 Phosphoserine.
 MOD_RES 645 645 Phosphothreonine (By similarity).
 MOD_RES 652 652 Phosphoserine.
 MOD_RES 654 654 Phosphothreonine.
 MOD_RES 655 655 Phosphoserine.
 MOD_RES 658 658 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Membrane; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 906 AA 

Protein Sequence

Gene Ontology

 GO:0001669; C:acrosomal vesicle; IEA:Compara.
 GO:0015629; C:actin cytoskeleton; IEA:InterPro.
 GO:0016342; C:catenin complex; IEA:Compara.
 GO:0014704; C:intercalated disc; IDA:MGI.
 GO:0030027; C:lamellipodium; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0005915; C:zonula adherens; IDA:MGI.
 GO:0051015; F:actin filament binding; IDA:MGI.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0007568; P:aging; IEA:Compara.
 GO:0043297; P:apical junction assembly; IMP:MGI.
 GO:0031103; P:axon regeneration; IEA:Compara.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0071681; P:cellular response to indole-3-methanol; IEA:Compara.
 GO:0007163; P:establishment or maintenance of cell polarity; IMP:MGI.
 GO:0016264; P:gap junction assembly; IEA:Compara.
 GO:0008584; P:male gonad development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0007406; P:negative regulation of neuroblast proliferation; IMP:MGI.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0001541; P:ovarian follicle development; IEA:Compara.
 GO:0045880; P:positive regulation of smoothened signaling pathway; IGI:MGI.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0043627; P:response to estrogen stimulus; IEA:Compara. 

Interpro

 IPR001033; Alpha_catenin.
 IPR006077; Vinculin/catenin.
 IPR000633; Vinculin_CS. 

Pfam

 PF01044; Vinculin 

SMART

  

PROSITE

 PS00663; VINCULIN_1 

PRINTS

 PR00805; ALPHACATENIN.