UniProt Accession

 LRIF1_HUMAN; Q5T3J3; Q86XS4; Q8N3B6; Q96HT4; Q9NUM5; Q9NV32 

Genbank Protein ID

 AY190122; AK001826; AK002131; AL360270; BC008115; AL834466 

Genbank Nucleotide ID

 AAO43631.1; BAA91928.1; BAA92097.1; CAI17128.1; AAH08115.1; CAD39125.1 

Protein Name

 Ligand-dependent nuclear receptor-interacting factor 1 

Protein Synonyms/Alias

 Receptor-interacting factor 1 

Gene Name

 LRIF1 

Gene Synonyms/Alias

 C1orf103; RIF1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	NLRRVFLKPAEENSG	ubiquitination	[1, 2, 3, 4, 5, 6]
50	LQLLPIPKSSGNLIP	ubiquitination	[5]
69	SVMSDALKGNTGKPV	ubiquitination	[1]
134	NFSSSVSKVQSHGVK	ubiquitination	[4, 5, 6]
175	QSLPVTVKSPVLPSG	ubiquitination	[5]
252	NFQNIYPKPVTEIAK	ubiquitination	[5]
259	KPVTEIAKPVILNTT	ubiquitination	[5, 6]
279	VATETQLKGGQHSQA	ubiquitination	[1, 2, 5, 6]
290	HSQAAPVKWIFQDNL	acetylation	[7]
290	HSQAAPVKWIFQDNL	ubiquitination	[4, 5, 6]
308	TPSLVPVKSSNNVAS	ubiquitination	[2, 5]
316	SSNNVASKILKTFVD	ubiquitination	[5]
319	NVASKILKTFVDRKN	ubiquitination	[5, 6]
325	LKTFVDRKNLGDNTI	ubiquitination	[4, 5]
348	DPSGTRSKNMPIKDN	ubiquitination	[5]
353	RSKNMPIKDNALVMF	ubiquitination	[5, 6]
369	GKVYLLAKKGTDVLP	ubiquitination	[2]
370	KVYLLAKKGTDVLPS	ubiquitination	[5, 6]
393	SPDTPVRKDTLQTVS	ubiquitination	[4, 5, 6]
418	VVNIVLAKSKSSQME	ubiquitination	[5]
427	KSSQMETKSLSNTQL	ubiquitination	[5, 6]
446	NLRAEKNKVEKPSPS	ubiquitination	[2, 5]
449	AEKNKVEKPSPSTTN	ubiquitination	[5]
470	SNYLKQSKTLFTNPI	ubiquitination	[2, 5]
491	TGHNAPRKVTAVIYA	ubiquitination	[5]
500	TAVIYARKGSVLQSI	ubiquitination	[5]
532	VFRDQEPKIHNEMAS	ubiquitination	[5]
543	EMASTSDKGAQGRND	ubiquitination	[1, 2, 3, 5]
565	SNKALHLKSDAEFKK	ubiquitination	[5, 6]
578	KKIFGLTKDLRVCLT	ubiquitination	[5]
605	DSFSSLVKSGTYKET	ubiquitination	[1, 2, 5, 6]
610	LVKSGTYKETEFMVK	ubiquitination	[5, 6]
617	KETEFMVKEGERKQQ	ubiquitination	[6]
705	YTHEKQEKGTLNSNA	ubiquitination	[5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Represses the ligand-induced transcriptional activity of retinoic acid receptor alpha (RARA). This repression may occur through direct recruitment of histone deacetylases. 

Sequence Annotation

 MOTIF 628 631 Nuclear localization signal.
 MOTIF 642 645 Nuclear localization signal.
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 599 599 Phosphoserine.
 MOD_RES 732 732 Phosphothreonine.  

Keyword

 Alternative splicing; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 769 AA 

Protein Sequence

Gene Ontology

 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR026191; LRIF1. 

Pfam

  

SMART

  

PROSITE

  

PRINTS