CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030565
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mortality factor 4-like protein 1 
Protein Synonyms/Alias
 cDNA FLJ38504 fis, clone HCHON2000156, highly similar to Mortality factor 4-like protein 1 
Gene Name
 MORF4L1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
89VPESRVLKYVDTNLQubiquitination[1, 2]
97YVDTNLQKQRELQKAubiquitination[1, 3, 4, 5, 6, 7]
103QKQRELQKANQEQYAubiquitination[2, 4, 5, 6, 7]
113QEQYAEGKMRGAAPGubiquitination[2, 4, 6, 7]
129KTSGLQQKNVEVKTKubiquitination[2, 4, 6, 7]
134QQKNVEVKTKKNKQKubiquitination[6]
190VKIPEELKPWLVDDWubiquitination[7]
204WDLITRQKQLFYLPAubiquitination[6]
212QLFYLPAKKNVDSILubiquitination[1, 4, 6, 7, 8, 9]
213LFYLPAKKNVDSILEubiquitination[6]
226LEDYANYKKSRGNTDubiquitination[1, 5, 7]
227EDYANYKKSRGNTDNubiquitination[1, 2, 6]
235SRGNTDNKEYAVNEVubiquitination[1, 4, 7, 8]
325DFLKYLAKNSATLFSubiquitination[9]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic snapshot of the EGF-induced ubiquitin network.
 Argenzio E, Bange T, Oldrini B, Bianchi F, Peesari R, Mari S, Di Fiore PP, Mann M, Polo S.
 Mol Syst Biol. 2011 Jan 18;7:462. [PMID: 21245847]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 348 AA 
Protein Sequence
MIPFPHPQVT DALGRYSNYI LSSTVESPSL RSMNFEKQGE RVLCFHGPLL YEAKCVKVAI 60
KDKQVKYFIH YSGWNKNWDE WVPESRVLKY VDTNLQKQRE LQKANQEQYA EGKMRGAAPG 120
KKTSGLQQKN VEVKTKKNKQ KTPGNGDGGS TSETPQPPRK KRARVDPTVE NEETFMNRVE 180
VKVKIPEELK PWLVDDWDLI TRQKQLFYLP AKKNVDSILE DYANYKKSRG NTDNKEYAVN 240
EVVAGIKEYF NVMLGTQLLY KFERPQYAEI LADHPDAPMS QVYGAPHLLR LFVRIGAMLA 300
YTPLDEKSLA LLLNYLHDFL KYLAKNSATL FSASDYEVAP PEYHRKAV 348 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro. 
Interpro
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR017398; EAF3/MRG15.
 IPR008676; MRG.
 IPR026541; MRG_dom.
 IPR025995; Tudor-knot. 
Pfam
 PF05712; MRG
 PF11717; Tudor-knot 
SMART
 SM00298; CHROMO 
PROSITE
 PS51640; MRG 
PRINTS