CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016758
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin-2 
Protein Synonyms/Alias
 ANK-2; Brain ankyrin 
Gene Name
 Ank2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
46ARAGNLDKVVEYLKGubiquitination[1]
52DKVVEYLKGGIDINTubiquitination[1]
118EVVKVLVKEGANINAubiquitination[1]
223HNADVQSKMMVNRTTubiquitination[1]
275TPLHVASKRGNTNMVubiquitination[1]
330APLLARTKNGLSPLHubiquitination[1]
382CGHYRVTKLLLDKRAubiquitination[1]
572TPLHVAAKYGSLDVAubiquitination[1]
638TPLHIAAKKNQMQIAubiquitination[1]
749KMVNFLLKQGANVNAubiquitination[1]
802NTALAIAKRLGYISVubiquitination[1]
938HQVSALAKEAERNSYubiquitination[1]
1180VKKILGNKATFSPIVubiquitination[1]
1302AKFVVFAKSHDPIEAubiquitination[1]
1383NRLPLFVKVRDTTQEubiquitination[1]
3571DQSHALLKYWLERDGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Attaches integral membrane proteins to cytoskeletal elements. Also binds to cytoskeletal proteins. Required for coordinate assembly of Na/Ca exchanger, Na/K ATPase and InsP3 receptor at sarcoplasmic reticulum sites in cardiomyocytes (By similarity). Required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) in the inner segment of rod photoreceptors. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. 
Sequence Annotation
 REPEAT 30 62 ANK 1.
 REPEAT 63 92 ANK 2.
 REPEAT 96 125 ANK 3.
 REPEAT 129 158 ANK 4.
 REPEAT 162 191 ANK 5.
 REPEAT 193 220 ANK 6.
 REPEAT 232 261 ANK 7.
 REPEAT 265 294 ANK 8.
 REPEAT 298 327 ANK 9.
 REPEAT 331 360 ANK 10.
 REPEAT 364 393 ANK 11.
 REPEAT 397 426 ANK 12.
 REPEAT 430 459 ANK 13.
 REPEAT 463 492 ANK 14.
 REPEAT 496 525 ANK 15.
 REPEAT 529 558 ANK 16.
 REPEAT 562 591 ANK 17.
 REPEAT 595 624 ANK 18.
 REPEAT 628 657 ANK 19.
 REPEAT 661 690 ANK 20.
 REPEAT 694 723 ANK 21.
 REPEAT 727 756 ANK 22.
 REPEAT 760 789 ANK 23.
 REPEAT 793 822 ANK 24.
 DOMAIN 966 1091 ZU5 1.
 DOMAIN 1092 1255 ZU5 2.
 DOMAIN 1417 1502 Death 1.
 DOMAIN 3522 3606 Death 2.
 REGION 966 1125 Interaction with SPTBN1 (By similarity).
 REGION 1256 1390 UPA domain (By similarity).
 MOD_RES 31 31 Phosphoserine.
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 378 378 Phosphotyrosine.
 MOD_RES 531 531 Phosphotyrosine.
 MOD_RES 846 846 Phosphoserine.
 MOD_RES 898 898 Phosphoserine.
 MOD_RES 1349 1349 Phosphotyrosine.
 MOD_RES 1428 1428 Phosphoserine.
 MOD_RES 3050 3050 Phosphothreonine.
 MOD_RES 3226 3226 Phosphoserine.
 MOD_RES 3315 3315 Phosphoserine.
 MOD_RES 3343 3343 Phosphoserine.
 MOD_RES 3362 3362 Phosphoserine.
 MOD_RES 3363 3363 Phosphotyrosine.
 MOD_RES 3686 3686 Phosphoserine.
 MOD_RES 3692 3692 Phosphoserine.
 MOD_RES 3718 3718 Phosphothreonine.
 MOD_RES 3744 3744 Phosphoserine.
 MOD_RES 3745 3745 Phosphothreonine.
 MOD_RES 3764 3764 Phosphoserine.
 MOD_RES 3785 3785 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; ANK repeat; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 3898 AA 
Protein Sequence
MMNEDAAQKS DSGEKFNGSS QRRKRPKKSD SNASFLRAAR AGNLDKVVEY LKGGIDINTC 60
NQNGLNALHL AAKEGHVGLV QELLGRGSSV DSATKKGNTA LHIASLAGQA EVVKVLVKEG 120
ANINAQSQNG FTPLYMAAQE NHIDVVKYLL ENGANQSTAT EDGFTPLAVA LQQGHNQAVA 180
ILLENDTKGK VRLPALHIAA RKDDTKSAAL LLQNDHNADV QSKMMVNRTT ESGFTPLHIA 240
AHYGNVNVAT LLLNRGAAVD FTARNGITPL HVASKRGNTN MVKLLLDRGG QIDAKTRDGL 300
TPLHCAARSG HDQVVELLLE RKAPLLARTK NGLSPLHMAA QGDHVECVKH LLQYKAPVDD 360
VTLDYLTALH VAAHCGHYRV TKLLLDKRAN PNARALNGFT PLHIACKKNR IKVMELLVKY 420
GASIQAITES GLTPIHVAAF MGHLNIVLLL LQNGASPDVT NIRGETALHM AARAGQVEVV 480
RCLLRNGALV DARAREEQTP LHIASRLGKT EIVQLLLQHM AHPDAATTNG YTPLHISARE 540
GQVDVASVLL EAGAAHSLAT KKGFTPLHVA AKYGSLDVAK LLLQRRAAAD SAGKNGLTPL 600
HVAAHYDNQK VALLLLEKGA SPHATAKNGY TPLHIAAKKN QMQIASTLLN YGAETNTVTK 660
QGVTPLHLAS QEGHTDMVTL LLDKGANIHM STKSGLTSLH LAAQEDKVNV ADILTKHGAD 720
RDAYTKLGYT PLIVACHYGN VKMVNFLLKQ GANVNAKTKN GYTPLHQAAQ QGHTHIINVL 780
LQHGAKPNAT TANGNTALAI AKRLGYISVV DTLKVVTEEV TTTTTTITEK HKLNVPETMT 840
EVLDVSDEEG DDTVTGDGGE YLRPEDLKEL GDDSLPSSQF LDGMNYLRYS LEGGRSDSLR 900
SFSSDRSHTL SHASYLRDSA MIDDTVVIPS HQVSALAKEA ERNSYRLSWG TENLDNVALS 960
SSPIHSGFLV SFMVDARGGA MRGCRHNGLR IIIPPRKCTA PTRVTCRLVK RHRLATMPPM 1020
VEGEGLASRL IEVGPSGAQF LGPVIVEIPH FAALRGKERE LVVLRSENGD SWKEHFCDYT 1080
EDELNEILNG MDEVLDSPED LEKKRICRII TRDFPQYFAV VSRIKQDSNL IGPEGGVLSS 1140
TVVSQVQAVF PEGALTKRIR VGLQAQPMHS ELVKKILGNK ATFSPIVTLE PRRRKFHKPI 1200
TMTIPVPKAS SDVMLNGFGG DAPTLRLLCS ITGGTTPAQW EDITGTTPLT FVNECVSFTT 1260
NVSARFWLID CRQIQESVAF ASQVYREIIC VPYMAKFVVF AKSHDPIEAR LRCFCMTDDK 1320
VDKTLEQQEN FSEVARSRDV EVLEGKPIYV DCFGNLVPLT KSGQHHIFSF FAFKENRLPL 1380
FVKVRDTTQE PCGRLSFMKE PKSTRGLVHQ AICNLNITLP IYAKESESDQ EPEEEIGMTS 1440
EKNDETESTE TSVLKSHLVN EVPVLASPDL LSEVSEMKQD LIKMTAILTT DVSDKAGSLK 1500
VKELAKAGEE EPGEPFEIVE RVKEDLEKVN AILRSGTCMR DEGRARSSQS ERELEEEWVI 1560
VSDEEIQEAK QHAPVEIDEH PCIEVRVDRE TKAKVEKDST GLVNYLTDDL NSYTSPHEKK 1620
PHTAPEKSGE TSQASAVGKS SESNKGKATS AEEKQSAQKQ LKPGLAIKKP VRRKLKEKQK 1680
QKEESSQSSE EKTELKKGSS EESVDEDRGL VPEPLPTAKA TSPLIEETPI GSIKDKVKAL 1740
QKRVEDEQKG RSKLPVRVKG KEDVPKRTTP RTHPAVSPSS KSSTSSKAER HSSLSSSAKP 1800
ERHTPVSPSS KNEKLSPVSP SAKTERHSPV FSGKPEKHSP GSPSTKNERH SPVSSLKTER 1860
HTPGSPSGKT DKRPPVPSSG RTEKHPPVSP GKTEKHLPGS PSIRTPEKPA PGSATGKHEK 1920
HLPVSPGKTE KQPPISPTSK TERIEETMSV RELMKAFQSG QDPSKHKTGL FEHKSAKQKQ 1980
PQDKSKSRVE KEKGHTVTQR EVTQRETQRI ESQTAKRGQR FQVSAATESR RFRSTTITVG 2040
LRMEDPVRER FERTPIIKTP EVVPSVAAEE SHRGSEKIVD EQGDMDFQIS PDRKTSTDFS 2100
EVIKQELEDN DKYQQFRLTE DTEKAQVHLD QVITSPFNTA FPLDYMKDEF LPALSLQSGA 2160
LGGSSESLKQ EVIAGSPCSS LMEGTPQISS EESYKHEGLA ETPETSPESL SFSPKKSEEQ 2220
IGEAKETTKV GTPTDIHSEK ELPITNDITD SSQKQGAGVT RGSEPSTEHS QKEVTQDPHK 2280
DVCSKQDGCP ESQSVSLASE VFTEKGSCGE SQLPLVSSAF KTQSESETQE SLTPSEVTKP 2340
FPPSDASVKT AEGTEPKPQG AIRSPQGLEL PLPNRDSEVL SPMADESLAV SHKDSLEASP 2400
VLEDNSSHKT PDSLEPSPLK ESPCRDSLES SPVEPKMKAG ILPSHFPLPA AIAKTDLVAE 2460
VASMRSRLLR DPDGSAEDDS LEQTSLMESS GKSPLSPDTP SSEEVSYEVT PKPSDSSTPK 2520
PAVIHECAEE DDSENGEKKR FTPEEEMFKM VTKIKTFDEL EQEAKQKRDY KKEPRQDGSS 2580
SASDPDADYS AEVNDEKQMA GTEGEGEVPV LVTSENRKVS SSSSESEPEL TQLSKGADSG 2640
LLTEPVIRVQ PPSPLPSSID SNSSPEEATQ FQPIVPKQYT FKMNEEIQEE PATSEDKDCK 2700
SHLAEDSQTH SADAADGSDG DLNRETTQPE TCDGHGCETV SPSNSATPVS LGVQSPEHKD 2760
VDKPLAIDKD SLAHQDTCEN DREEREFDPS GVESTQADLP NESSSLSSRC AIPEGNESAK 2820
EIASPSSPVK VEVTITDQAL ESMPEDCPIQ DSSTTMQTER FAMDVPVSEL AETDENSDPQ 2880
IISPYENVPS SSFFSAEPSK IQTDTCHSTV VHSPEVYSVI IRSSPEDVVV TNSSNRTVSG 2940
EESHCESHDL ETESEQKSAL WAAQSDAPPL AVAPTASDAA SVTGEQASKV IITKTDADAD 3000
SWSEIREDDA AFEARVKEEE QKIFGLMVDR QSQGTTPDTT PARTPTEEGT PTSEQNPFLF 3060
QEGKLFEMTR SGAIDMTKRP YADESLHFFQ IGQESNEEAI SEDLKEGATG AEPPQTETTS 3120
ESLELSEPKE AMDDEGELLP DDVSEEIEDL PASDANIDSQ VIISASTETP TKEAVSTAVE 3180
EPPTTQRSDS LSTVKQTPRP AVPGPVGQLD FSPVTRSVYS GQDDESPESS PEEQKSVIEI 3240
PTAPVDNVPS AESKPQIPIR TLPTLVPAPP SAEDESAFSD DFPSSLDEDS KEGGAKPKSK 3300
IPVKAPTQRT EWQPSPTDIP LQKTAVPQGQ ETLSRAPDGR SKSESDASSL DAKTKCPVKA 3360
RSYIETETES RERAEGFESE SEDGATKPKL FASRLPVKSR STSSSGRPGT SPTRESREHF 3420
FDLYRNSIEF FEEISDEASK LVDRLTQSER EQEPPSDDES SSALEVSVIE SLPPVDIEHS 3480
APEDIFDTRP IWDESIETMI ERIPDENGHD RAEDPQDEQE RMEERLAYIA DHLGFSWTEL 3540
ARELDFTEEQ IHQIRIENPN SLQDQSHALL KYWLERDGKH ATDTILIECL TKINRMDIVH 3600
LLETNTEPLQ ERMGRSYAEI EQTITLDHSE GFSVLPDELC AAKEKKEQEA SKESESSDHP 3660
PMVSEEDISV GYSTFQDCLP KTEGDSPAAA LSPQMHQEPV QQDFSGKTQD QQEYYVTTPG 3720
AEVEDPQKAT AVPDSLCKTP EDISTPPEGT KPCLQTPVTS ERGSPIVQEP EEASEPKEES 3780
SPRKTSLVIV ESTDDQSQVF ERLDGDAAFQ KGDDMPDIPP ETVTEEEYVD ENGHTVVKKV 3840
TRKIIRRYVS SDGTEKEEVT MQGMPQEPVN IEDGDNYSKV IKRVVLKSDT QQSEDNNE 3898 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0043034; C:costamere; IDA:BHF-UCL.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; IDA:UniProtKB.
 GO:0014704; C:intercalated disc; IDA:BHF-UCL.
 GO:0031430; C:M band; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0030315; C:T-tubule; IDA:BHF-UCL.
 GO:0030018; C:Z disc; IDA:BHF-UCL.
 GO:0015459; F:potassium channel regulator activity; IMP:MGI.
 GO:0030674; F:protein binding, bridging; IDA:BHF-UCL.
 GO:0003283; P:atrial septum development; IEA:Compara.
 GO:0060048; P:cardiac muscle contraction; IMP:MGI.
 GO:0006874; P:cellular calcium ion homeostasis; IMP:BHF-UCL.
 GO:1901021; P:positive regulation of calcium ion transmembrane transporter activity; IMP:BHF-UCL.
 GO:0051928; P:positive regulation of calcium ion transport; IMP:BHF-UCL.
 GO:2001259; P:positive regulation of cation channel activity; IMP:BHF-UCL.
 GO:0010628; P:positive regulation of gene expression; IMP:BHF-UCL.
 GO:1901018; P:positive regulation of potassium ion transmembrane transporter activity; IMP:BHF-UCL.
 GO:0043268; P:positive regulation of potassium ion transport; IMP:BHF-UCL.
 GO:0034394; P:protein localization to cell surface; IMP:UniProtKB.
 GO:0070972; P:protein localization to endoplasmic reticulum; IMP:BHF-UCL.
 GO:0036309; P:protein localization to M-band; IMP:BHF-UCL.
 GO:0036371; P:protein localization to T-tubule; IMP:BHF-UCL.
 GO:0050821; P:protein stabilization; IMP:BHF-UCL.
 GO:0072661; P:protein targeting to plasma membrane; IMP:MGI.
 GO:0086014; P:regulation of atrial cardiac muscle cell action potential; IMP:BHF-UCL.
 GO:0010881; P:regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion; IMP:BHF-UCL.
 GO:0086091; P:regulation of heart rate by cardiac conduction; IMP:BHF-UCL.
 GO:0086015; P:regulation of SA node cell action potential; IMP:BHF-UCL.
 GO:0086005; P:regulation of ventricular cardiac muscle cell action potential; IEA:Compara.
 GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; IEA:Compara.
 GO:0033292; P:T-tubule organization; IMP:BHF-UCL. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR011029; DEATH-like_dom.
 IPR000488; Death_domain.
 IPR000906; ZU5. 
Pfam
 PF00023; Ank
 PF12796; Ank_2
 PF00531; Death
 PF00791; ZU5 
SMART
 SM00248; ANK
 SM00005; DEATH
 SM00218; ZU5 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50017; DEATH_DOMAIN
 PS51145; ZU5 
PRINTS
 PR01415; ANKYRIN.