CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025772
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MKIAA4040 protein 
Protein Synonyms/Alias
  
Gene Name
 Rapgef4 
Gene Synonyms/Alias
 mKIAA4040 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
226ITKVPSEKILRAGKIubiquitination[1]
307QERHFQDKYLFYRFLubiquitination[1]
655PELEKIVKQISEDAKubiquitination[1]
669KAPQKKHKVLLQQFNubiquitination[1]
717VPVAASVKEVISAVAubiquitination[1]
939AYRLTAAKLEPPLIPubiquitination[1]
974DNLVNFEKMRMIANTubiquitination[1]
1002PDAAQANKNHQDVRSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 Guanine-nucleotide releasing factor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1035 AA 
Protein Sequence
GRGSPPGGAR EVAAARGHRA LLPNMVAAHA AHSQSSAEWI ACLDKRPLER SSEDVDIIFT 60
RLKGVKAFEK FHPNLLRQIC LCGYYENLEK GITLFRQGDI GTNWYAVLAG SLDVKVSETS 120
SHQDAVTICT LGIGTAFGES ILDNTPRHAT IVTRESSELL RIEQEDFKAL WEKYRQYMAG 180
LLAPPYGVME TGSNNDRIPD KENTPLIEPH VPLRPAHTIT KVPSEKILRA GKILRIAILS 240
RAPHMIRDRK YHLKTYRQCC VGTELVDWMI QQTSCVHSRT QAVGMWQVLL EDGVLNHVDQ 300
ERHFQDKYLF YRFLDDERED APLPTEEEKK ECDEELQDTM LLLSQMGPDA HMRMILRKPP 360
GQRTVDDLEI IYDELLHIKA LSHLSTTVKR ELAGVLIFES HAKGGTVLFN QGEEGTSWYI 420
ILKGSVNVVI YGKGVVCTLH EGDDFGKLAL VNDAPRAASI VLREDNCHFL RVDKEDFNRI 480
LRDVEANTVR LKEHDQDVLV LEKVPAGNRA ANQGNSQPQQ KYTVMSGTPE KILEHFLETI 540
RLEPSLNEAT DSVLNDFVMM HCVFMPNTQL CPALVAHYHA QPSQGTEQER MDYALNNKRR 600
VIRLVLQWAA MYGDLLQEDD VAMAFLEEFY VSVSDDARMM AAFKEQLPEL EKIVKQISED 660
AKAPQKKHKV LLQQFNTGDE RAQKRQPIRG SDEVLFKVYC IDHTYTTIRV PVAASVKEVI 720
SAVADKLGSG EGLIIVKMNS GGEKVVLKSN DVSVFTTLTI NGRLFACPRE QFDSLTPLPE 780
QEGPTTGTVG TFELMSSKDL AYQMTTYDWE LFNCVHELEL IYHTFGRHNF KKTTANLDLF 840
LRRFNEIQFW VVTEVCLCSQ LSKRVQLLKK FIKIAAHCKE YKNLNSFFAI VMGLSNVAVS 900
RLALTWEKLP SKFKKFYAEF ESLMDPSRNH RAYRLTAAKL EPPLIPFMPL LIKDMTFTHE 960
GNKTFIDNLV NFEKMRMIAN TARTVRYYRS QPFNPDAAQA NKNHQDVRSY VRQLNVIDNQ 1020
RTLSQMSHRL EPRRP 1035 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:MGI.
 GO:0017156; P:calcium ion-dependent exocytosis; IDA:MGI.
 GO:0019933; P:cAMP-mediated signaling; IDA:MGI.
 GO:0030073; P:insulin secretion; IDA:MGI.
 GO:0017157; P:regulation of exocytosis; IDA:MGI.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR002373; cAMP/cGMP_kin.
 IPR018490; cNMP-bd-like.
 IPR000595; cNMP-bd_dom.
 IPR000591; DEP_dom.
 IPR000159; Ras-assoc.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR019804; Ras_G-nucl-exch_fac_CS.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25.
 IPR014710; RmlC-like_jellyroll.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00027; cNMP_binding
 PF00610; DEP
 PF00788; RA
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00100; cNMP
 SM00049; DEP
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS50042; CNMP_BINDING_3
 PS50186; DEP
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER 
PRINTS
 PR00103; CAMPKINASE.