UniProt Accession

 DPOLB_HUMAN; P06746; B2RC78; Q3KP48; Q6FI34 

Genbank Protein ID

 L11607; D29013; U10526; U10516; U10517; U10519; U10520; U10521; U10522; U10523; U10524; U10525; AK314976; CR536503; CR541802; AF491812; BC100288; BC106909; J04201; M13140 

Genbank Nucleotide ID

 AAB59441.1; BAA06099.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; AAB60688.1; BAG37475.1; CAG38741.1; CAG46601.1; AAL91594.1; AAI00289.1; AAI06910.1; AAA60134.1; AAA60133.1 

Protein Name

 DNA polymerase beta 

Protein Synonyms/Alias

  

Gene Name

 POLB 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
41	HKYNAYRKAASVIAK	ubiquitination	[1]
61	KSGAEAKKLPGVGTK	ubiquitination	[1]
72	VGTKIAEKIDEFLAT	acetylation	[2]
81	DEFLATGKLRKLEKI	ubiquitination	[1]
113	IGPSAARKFVDEGIK	ubiquitination	[3]
141	HHQRIGLKYFGDFEK	ubiquitination	[3]
148	KYFGDFEKRIPREEM	ubiquitination	[3]
230	FITDTLSKGETKFMG	ubiquitination	[3]

Reference

 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Acetylation regulates the DNA end-trimming activity of DNA polymerase beta.
 Hasan S, El-Andaloussi N, Hardeland U, Hassa PO, Bürki C, Imhof R, Schär P, Hottiger MO.
 Mol Cell. 2002 Nov;10(5):1213-22. [PMID: 12453427]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. 

Sequence Annotation

 REGION 183 192 DNA binding.
 ACT_SITE 72 72 Schiff-base intermediate with DNA.
 METAL 101 101 Sodium; via carbonyl oxygen.
 METAL 103 103 Sodium; via carbonyl oxygen.
 METAL 106 106 Sodium; via carbonyl oxygen.
 METAL 190 190 Magnesium 1.
 METAL 190 190 Magnesium 2.
 METAL 192 192 Magnesium 1.
 METAL 192 192 Magnesium 2.
 METAL 256 256 Magnesium 2.
 MOD_RES 83 83 Omega-N-methylarginine; by PRMT6.
 MOD_RES 152 152 Omega-N-methylarginine; by PRMT6.
 CROSSLNK 41 41 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 61 61 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 81 81 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Complete proteome; Cytoplasm; DNA damage; DNA repair; DNA replication; DNA synthesis; DNA-binding; DNA-directed DNA polymerase; Isopeptide bond; Lyase; Magnesium; Metal-binding; Methylation; Nucleotidyltransferase; Nucleus; Polymorphism; Reference proteome; Sodium; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 335 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005876; C:spindle microtubule; IDA:MGI.
 GO:0003684; F:damaged DNA binding; IEA:Compara.
 GO:0003887; F:DNA-directed DNA polymerase activity; IDA:UniProtKB.
 GO:0016829; F:lyase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; IDA:MGI.
 GO:0006284; P:base-excision repair; IDA:UniProtKB.
 GO:0008219; P:cell death; IEA:Compara.
 GO:0006261; P:DNA-dependent DNA replication; TAS:ProtInc.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0006290; P:pyrimidine dimer repair; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara. 

Interpro

 IPR002054; DNA-dir_DNA_pol_X.
 IPR019843; DNA_pol-X_BS.
 IPR010996; DNA_pol_b-like_N.
 IPR018944; DNA_pol_lambd_fingers_domain.
 IPR022312; DNA_pol_X.
 IPR002008; DNA_pol_X_beta-like.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR027420; PolB_N. 

Pfam

 PF10391; DNA_pol_lambd_f 

SMART

 SM00278; HhH1
 SM00483; POLXc 

PROSITE

 PS00522; DNA_POLYMERASE_X 

PRINTS

 PR00869; DNAPOLX.
 PR00870; DNAPOLXBETA.