CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006462
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sepiapterin reductase 
Protein Synonyms/Alias
 SPR 
Gene Name
 SPR 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
230QELKAKGKLVDCKVSacetylation[1]
240DCKVSAQKLLSLLEKubiquitination[2, 3, 4]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the final one or two reductions in tetra- hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin. 
Sequence Annotation
 NP_BIND 14 20 NADP.
 NP_BIND 42 43 NADP.
 NP_BIND 69 70 NADP.
 NP_BIND 201 206 NADP.
 REGION 157 158 Substrate binding (By similarity).
 BINDING 170 170 Substrate (By similarity).
 BINDING 174 174 NADP (By similarity).
 BINDING 199 199 Substrate; via amide nitrogen (By
 BINDING 257 257 Substrate (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 213 213 Phosphoserine; by CaMK2; in vitro.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Disease mutation; Dystonia; NADP; Oxidoreductase; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 261 AA 
Protein Sequence
MEGGLGRAVC LLTGASRGFG RTLAPLLASL LSPGSVLVLS ARNDEALRQL EAELGAERSG 60
LRVVRVPADL GAEAGLQQLL GALRELPRPK GLQRLLLINN AGSLGDVSKG FVDLSDSTQV 120
NNYWALNLTS MLCLTSSVLK AFPDSPGLNR TVVNISSLCA LQPFKGWALY CAGKAARDML 180
FQVLALEEPN VRVLNYAPGP LDTDMQQLAR ETSVDPDMRK GLQELKAKGK LVDCKVSAQK 240
LLSLLEKDEF KSGAHVDFYD K 261 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004033; F:aldo-keto reductase (NADP) activity; TAS:UniProtKB.
 GO:0050661; F:NADP binding; TAS:UniProtKB.
 GO:0004757; F:sepiapterin reductase activity; ISS:UniProtKB.
 GO:0006809; P:nitric oxide biosynthetic process; IDA:UniProtKB.
 GO:0050999; P:regulation of nitric-oxide synthase activity; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006729; P:tetrahydrobiopterin biosynthetic process; TAS:UniProtKB. 
Interpro
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR006393; Sepiapterin_red. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
  
PRINTS
 PR00081; GDHRDH.