UniProt Accession

 IMDH2_MOUSE; P24547; Q61734; Q91Z11 

Genbank Protein ID

 M33934; M98333; BC010314; BC052671 

Genbank Nucleotide ID

 AAA39311.1; AAA20181.1; AAH10314.1; AAH52671.1 

Protein Name

 Inosine-5'-monophosphate dehydrogenase 2 

Protein Synonyms/Alias

 IMP dehydrogenase 2; IMPD 2; IMPDH 2; IMPDH-II 

Gene Name

 Impdh2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
109	NEVRKVKKYEQGFIT	acetylation	[1]
109	NEVRKVKKYEQGFIT	ubiquitination	[2]
134	VRDVFEAKARHGFCG	ubiquitination	[2]
195	APAGVTLKEANEILQ	ubiquitination	[2]
206	EILQRSKKGKLPIVN	ubiquitination	[2]
208	LQRSKKGKLPIVNEN	ubiquitination	[2]
242	LASKDAKKQLLCGAA	ubiquitination	[2]
257	IGTHEDDKYRLDLLA	acetylation	[3]
293	MIKYIKEKYPSLQVI	ubiquitination	[2]
450	VSGAVQDKGSIHKFV	ubiquitination	[2]
474	SCQDIGAKSLTQVRA	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (By similarity). It may also have a role in the development of malignancy and the growth progression of some tumors. 

Sequence Annotation

 DOMAIN 114 173 CBS 1.
 DOMAIN 179 237 CBS 2.
 NP_BIND 274 276 NAD (By similarity).
 NP_BIND 324 326 NAD (By similarity).
 REGION 364 366 IMP binding (By similarity).
 REGION 387 388 IMP binding (By similarity).
 REGION 411 415 IMP binding (By similarity).
 ACT_SITE 331 331 Thioimidate intermediate (By similarity).
 METAL 326 326 Potassium; via carbonyl oxygen (By
 METAL 328 328 Potassium; via carbonyl oxygen (By
 METAL 331 331 Potassium; via carbonyl oxygen (By
 METAL 500 500 Potassium; via carbonyl oxygen; shared
 METAL 501 501 Potassium; via carbonyl oxygen; shared
 METAL 502 502 Potassium; via carbonyl oxygen; shared
 BINDING 329 329 IMP (By similarity).
 BINDING 441 441 IMP (By similarity).
 MOD_RES 122 122 Phosphoserine (By similarity).
 MOD_RES 400 400 Phosphotyrosine (By similarity).
 MOD_RES 416 416 Phosphoserine (By similarity).
 MOD_RES 511 511 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; CBS domain; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; GMP biosynthesis; Metal-binding; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Potassium; Purine biosynthesis; Reference proteome; Repeat; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 514 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003938; F:IMP dehydrogenase activity; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:HAMAP.
 GO:0000166; F:nucleotide binding; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0071353; P:cellular response to interleukin-4; IDA:MGI.
 GO:0006177; P:GMP biosynthetic process; IEA:HAMAP.
 GO:0046651; P:lymphocyte proliferation; IMP:MGI.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0006164; P:purine nucleotide biosynthetic process; IMP:MGI.
 GO:0060041; P:retina development in camera-type eye; IEA:Compara. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR000644; Cysta_beta_synth_core.
 IPR005990; IMP_DH.
 IPR015875; IMP_DH/GMP_Rdtase_CS.
 IPR001093; IMP_DH_GMPRt. 

Pfam

 PF00571; CBS
 PF00478; IMPDH 

SMART

 SM00116; CBS 

PROSITE

 PS51371; CBS
 PS00487; IMP_DH_GMP_RED 

PRINTS