UniProt Accession

 MKRN2_HUMAN; Q9H000; A6NIA2; B3KRC5; Q8N391; Q96BD4; Q9BUY2; Q9NRY1 

Genbank Protein ID

 AF302084; AF277170; AF277164; AF277165; AF277166; AF277167; AF277168; AF277169; AF161555; AK091318; AC018500; CH471055; BC001799; BC015715; AL834512 

Genbank Nucleotide ID

 AAG30426.1; AAG27595.1; AAG27595.1; AAG27595.1; AAG27595.1; AAG27595.1; AAG27595.1; AAG27595.1; AAF29042.2; BAG52337.1; EAW64132.1; AAH01799.2; AAH15715.1; CAD39168.1 

Protein Name

 Probable E3 ubiquitin-protein ligase makorin-2 

Protein Synonyms/Alias

 RING finger protein 62 

Gene Name

 MKRN2 

Gene Synonyms/Alias

 RNF62; HSPC070 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MSTKQITCRYF	ubiquitination	[1, 2]
42	TICKYYQKGYCAYGT	ubiquitination	[2, 3]
143	PQPSPEMKPHSYLDA	ubiquitination	[2]
214	EQRKAHEKICMLTFE	ubiquitination	[2]
278	IRQWRCAKQFENPII	ubiquitination	[2, 3]
310	YWVEDQNKKNELIEA	ubiquitination	[1]
311	WVEDQNKKNELIEAF	ubiquitination	[2]
319	NELIEAFKQGMGKKA	ubiquitination	[2]
328	GMGKKACKYFEQGKG	ubiquitination	[2]
334	CKYFEQGKGTCPFGS	ubiquitination	[1, 2]
342	GTCPFGSKCLYRHAY	ubiquitination	[2]
359	GRLAEPEKPRKQLSS	ubiquitination	[2]
362	AEPEKPRKQLSSQGT	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). 

Sequence Annotation

 ZN_FING 2 29 C3H1-type 1.
 ZN_FING 31 58 C3H1-type 2.
 ZN_FING 165 192 C3H1-type 3.
 ZN_FING 238 292 RING-type.
 ZN_FING 321 350 C3H1-type 4.
 REGION 193 222 Makorin-type Cys-His.  

Keyword

 Complete proteome; Ligase; Metal-binding; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 416 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IDA:UniProtKB.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 

Interpro

 IPR026290; Makorin-related.
 IPR026293; Makorin_2.
 IPR000571; Znf_CCCH.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 

Pfam

 PF00642; zf-CCCH
 PF13639; zf-RING_2 

SMART

 SM00184; RING
 SM00356; ZnF_C3H1 

PROSITE

 PS50103; ZF_C3H1
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS