CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019669
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Niban-like protein 1 
Protein Synonyms/Alias
 Meg-3; Melanoma invasion by ERK; MINERVA; Protein FAM129B 
Gene Name
 FAM129B 
Gene Synonyms/Alias
 C9orf88 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
104GLVLYENKAAYERQVubiquitination[1, 2, 3, 4]
145SLPGTTAKSGSAPILubiquitination[1, 2, 4]
153SGSAPILKCPTQFPLubiquitination[2]
203NGIPEDSKVEGPAFTubiquitination[2]
293RFEEVLSKVQQVQPAubiquitination[4]
316MDQIITSKEHLASKIubiquitination[1, 2, 3, 4, 5, 6]
384INEGGIDKLGEYMEKubiquitination[1]
391KLGEYMEKLSRLAYHubiquitination[1]
401RLAYHPLKMQSCYEKubiquitination[2, 3, 5, 6]
430VSSTSVFKQRAQIHMubiquitination[1, 2, 3, 4, 5, 6, 7]
462ELGKGPTKEELCKSIubiquitination[2, 4]
507SIPFLLKKLAPTCKSubiquitination[2]
513KKLAPTCKSELPRFQubiquitination[2]
554KDILQAVKEAAVQRKubiquitination[1, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 May play a role in apoptosis suppression. May promote melanoma cell invasion in vitro. 
Sequence Annotation
 DOMAIN 68 192 PH.
 MOD_RES 568 568 Phosphoserine.
 MOD_RES 574 574 Phosphoserine.
 MOD_RES 601 601 Phosphoserine.
 MOD_RES 603 603 Phosphoserine.
 MOD_RES 606 606 Phosphothreonine.
 MOD_RES 609 609 Phosphoserine.
 MOD_RES 624 624 Phosphoserine.
 MOD_RES 638 638 Phosphoserine.
 MOD_RES 641 641 Phosphoserine.
 MOD_RES 646 646 Phosphoserine.
 MOD_RES 665 665 Phosphoserine.
 MOD_RES 681 681 Phosphoserine.
 MOD_RES 692 692 Phosphoserine.
 MOD_RES 696 696 Phosphoserine.  
Keyword
 Alternative splicing; Cell junction; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 746 AA 
Protein Sequence
MGDVLSTHLD DARRQHIAEK TGKILTEFLQ FYEDQYGVAL FNSMRHEIEG TGLPQAQLLW 60
RKVPLDERIV FSGNLFQHQE DSKKWRNRFS LVPHNYGLVL YENKAAYERQ VPPRAVINSA 120
GYKILTSVDQ YLELIGNSLP GTTAKSGSAP ILKCPTQFPL ILWHPYARHY YFCMMTEAEQ 180
DKWQAVLQDC IRHCNNGIPE DSKVEGPAFT DAIRMYRQSK ELYGTWEMLC GNEVQILSNL 240
VMEELGPELK AELGPRLKGK PQERQRQWIQ ISDAVYHMVY EQAKARFEEV LSKVQQVQPA 300
MQAVIRTDMD QIITSKEHLA SKIRAFILPK AEVCVRNHVQ PYIPSILEAL MVPTSQGFTE 360
VRDVFFKEVT DMNLNVINEG GIDKLGEYME KLSRLAYHPL KMQSCYEKME SLRLDGLQQR 420
FDVSSTSVFK QRAQIHMREQ MDNAVYTFET LLHQELGKGP TKEELCKSIQ RVLERVLKKY 480
DYDSSSVRKR FFREALLQIS IPFLLKKLAP TCKSELPRFQ ELIFEDFARF ILVENTYEEV 540
VLQTVMKDIL QAVKEAAVQR KHNLYRDSMV MHNSDPNLHL LAEGAPIDWG EEYSNSGGGG 600
SPSPSTPESA TLSEKRRRAK QVVSVVQDEE VGLPFEASPE SPPPASPDGV TEIRGLLAQG 660
LRPESPPPAG PLLNGAPAGE SPQPKAAPEA SSPPASPLQH LLPGKAVDLG PPKPSDQETG 720
EQVSSPSSHP ALHTTTEDSA GVQTEF 746 
Gene Ontology
 GO:0005912; C:adherens junction; IDA:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB. 
Interpro
 IPR026088; Niban_like.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
  
SMART
 SM00233; PH 
PROSITE
 PS50003; PH_DOMAIN 
PRINTS