UniProt Accession

 GPSM1_HUMAN; Q86YR5; A9Z1X4; B1B0W3; Q86SR5; Q969T1; Q9UFS8 

Genbank Protein ID

 AY173053; BX649589; BX649589; BX649589; CH471090; BC009979; BC017353; BC048343; AL117478 

Genbank Nucleotide ID

 AAO17260.1; CAI19338.1; CAI19339.1; CAM27654.1; EAW88216.1; AAH09979.1; AAH17353.1; AAH48343.1; CAB55951.1 

Protein Name

 G-protein-signaling modulator 1 

Protein Synonyms/Alias

 Activator of G-protein signaling 3 

Gene Name

 GPSM1 

Gene Synonyms/Alias

 AGS3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
90	GRALEYHKHDLLLAR	ubiquitination	[1]
107	GDRMGEAKASGNLGN	ubiquitination	[1, 2]
117	GNLGNTLKVLGRFDE	ubiquitination	[3, 4]
142	IAQEQGDKVGEARAL	ubiquitination	[1, 2]
187	DVRETLCKASEFYER	ubiquitination	[1]
200	ERNLSLVKELGDRAA	ubiquitination	[1]
233	TEATTFHKERLAIAK	ubiquitination	[1]
245	IAKEFGDKAAERRAY	ubiquitination	[1]
274	VAAEYYKKTLQLSRQ	ubiquitination	[1]
354	QALTFAKKHLQISQE	ubiquitination	[1]
393	TSPAASEKPDLAGYE	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G- protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction. 

Sequence Annotation

 REPEAT 28 61 TPR 1.
 REPEAT 66 99 TPR 2.
 REPEAT 106 139 TPR 3.
 REPEAT 146 181 TPR 4.
 REPEAT 183 202 TPR 5.
 REPEAT 209 242 TPR 6.
 REPEAT 249 282 TPR 7.
 REPEAT 289 322 TPR 8.
 REPEAT 329 362 TPR 9.
 DOMAIN 495 517 GoLoco 1.
 DOMAIN 548 570 GoLoco 2.
 DOMAIN 596 618 GoLoco 3.
 DOMAIN 630 652 GoLoco 4.
 REGION 1 509 Mediates association with membranes (By
 REGION 364 487 Interaction with STK11/LKB1 (By
 MOD_RES 413 413 Phosphoserine.
 MOD_RES 471 471 Phosphoserine.
 MOD_RES 492 492 Phosphoserine.
 MOD_RES 493 493 Phosphoserine.  

Keyword

 Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Endoplasmic reticulum; Golgi apparatus; Membrane; Neurogenesis; Phosphoprotein; Reference proteome; Repeat; TPR repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 675 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005092; F:GDP-dissociation inhibitor activity; IEA:InterPro.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; IEA:InterPro. 

Interpro

 IPR024804; G_prot_signal_mod_1.
 IPR003109; GoLoco_motif.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF02188; GoLoco
 PF00515; TPR_1
 PF13176; TPR_7 

SMART

 SM00390; GoLoco
 SM00028; TPR 

PROSITE

 PS50877; GOLOCO
 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS