CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010424
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein jagged-1 
Protein Synonyms/Alias
 Jagged1; hJ1; CD339 
Gene Name
 JAG1 
Gene Synonyms/Alias
 JAGL1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
728YDEGDAFKCMCPGGWubiquitination[1]
1123REQLNQIKNPIEKHGubiquitination[1, 2, 3, 4]
1128QIKNPIEKHGANTVPubiquitination[1]
1137GANTVPIKDYENKNSubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). 
Sequence Annotation
 DOMAIN 185 229 DSL.
 DOMAIN 230 263 EGF-like 1.
 DOMAIN 264 294 EGF-like 2; atypical.
 DOMAIN 296 334 EGF-like 3.
 DOMAIN 336 372 EGF-like 4.
 DOMAIN 374 410 EGF-like 5; calcium-binding (Potential).
 DOMAIN 412 448 EGF-like 6; calcium-binding (Potential).
 DOMAIN 450 485 EGF-like 7; calcium-binding (Potential).
 DOMAIN 487 523 EGF-like 8; calcium-binding (Potential).
 DOMAIN 525 561 EGF-like 9.
 DOMAIN 586 627 EGF-like 10.
 DOMAIN 629 665 EGF-like 11; calcium-binding (Potential).
 DOMAIN 667 703 EGF-like 12; calcium-binding (Potential).
 DOMAIN 705 741 EGF-like 13.
 DOMAIN 744 780 EGF-like 14.
 DOMAIN 782 818 EGF-like 15; calcium-binding (Potential).
 DOMAIN 820 856 EGF-like 16; calcium-binding (Potential).
 REGION 199 207 Important for interaction with NOTCH1.
 CARBOHYD 143 143 N-linked (GlcNAc...) (Potential).
 CARBOHYD 217 217 N-linked (GlcNAc...) (Potential).
 CARBOHYD 382 382 N-linked (GlcNAc...) (Potential).
 CARBOHYD 559 559 N-linked (GlcNAc...) (Potential).
 CARBOHYD 745 745 N-linked (GlcNAc...) (Potential).
 CARBOHYD 960 960 N-linked (GlcNAc...) (Potential).
 CARBOHYD 991 991 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1045 1045 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1064 1064 N-linked (GlcNAc...) (Potential).
 DISULFID 187 196
 DISULFID 200 212
 DISULFID 220 229
 DISULFID 234 245
 DISULFID 238 251
 DISULFID 253 262
 DISULFID 265 276
 DISULFID 271 282
 DISULFID 284 293
 DISULFID 300 312
 DISULFID 306 322
 DISULFID 324 333
 DISULFID 340 351 By similarity.
 DISULFID 345 360 By similarity.
 DISULFID 362 371 By similarity.
 DISULFID 378 389 By similarity.
 DISULFID 383 398 By similarity.
 DISULFID 400 409 By similarity.
 DISULFID 416 427 By similarity.
 DISULFID 421 436 By similarity.
 DISULFID 438 447 By similarity.
 DISULFID 454 464 By similarity.
 DISULFID 458 473 By similarity.
 DISULFID 475 484 By similarity.
 DISULFID 491 502 By similarity.
 DISULFID 496 511 By similarity.
 DISULFID 513 522 By similarity.
 DISULFID 529 540 By similarity.
 DISULFID 534 549 By similarity.
 DISULFID 551 560 By similarity.
 DISULFID 578 605 By similarity.
 DISULFID 599 615 By similarity.
 DISULFID 617 626 By similarity.
 DISULFID 633 644 By similarity.
 DISULFID 638 653 By similarity.
 DISULFID 655 664 By similarity.
 DISULFID 671 682 By similarity.
 DISULFID 676 691 By similarity.
 DISULFID 693 702 By similarity.
 DISULFID 709 720 By similarity.
 DISULFID 714 729 By similarity.
 DISULFID 731 740 By similarity.
 DISULFID 748 759 By similarity.
 DISULFID 753 768 By similarity.
 DISULFID 770 779 By similarity.
 DISULFID 786 797 By similarity.
 DISULFID 791 806 By similarity.
 DISULFID 808 817 By similarity.
 DISULFID 824 835 By similarity.
 DISULFID 829 844 By similarity.
 DISULFID 846 855 By similarity.  
Keyword
 3D-structure; Calcium; Complete proteome; Developmental protein; Disease mutation; Disulfide bond; EGF-like domain; Glycoprotein; Membrane; Notch signaling pathway; Polymorphism; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1218 AA 
Protein Sequence
MRSPRTRGRS GRPLSLLLAL LCALRAKVCG ASGQFELEIL SMQNVNGELQ NGNCCGGARN 60
PGDRKCTRDE CDTYFKVCLK EYQSRVTAGG PCSFGSGSTP VIGGNTFNLK ASRGNDRNRI 120
VLPFSFAWPR SYTLLVEAWD SSNDTVQPDS IIEKASHSGM INPSRQWQTL KQNTGVAHFE 180
YQIRVTCDDY YYGFGCNKFC RPRDDFFGHY ACDQNGNKTC MEGWMGPECN RAICRQGCSP 240
KHGSCKLPGD CRCQYGWQGL YCDKCIPHPG CVHGICNEPW QCLCETNWGG QLCDKDLNYC 300
GTHQPCLNGG TCSNTGPDKY QCSCPEGYSG PNCEIAEHAC LSDPCHNRGS CKETSLGFEC 360
ECSPGWTGPT CSTNIDDCSP NNCSHGGTCQ DLVNGFKCVC PPQWTGKTCQ LDANECEAKP 420
CVNAKSCKNL IASYYCDCLP GWMGQNCDIN INDCLGQCQN DASCRDLVNG YRCICPPGYA 480
GDHCERDIDE CASNPCLNGG HCQNEINRFQ CLCPTGFSGN LCQLDIDYCE PNPCQNGAQC 540
YNRASDYFCK CPEDYEGKNC SHLKDHCRTT PCEVIDSCTV AMASNDTPEG VRYISSNVCG 600
PHGKCKSQSG GKFTCDCNKG FTGTYCHENI NDCESNPCRN GGTCIDGVNS YKCICSDGWE 660
GAYCETNIND CSQNPCHNGG TCRDLVNDFY CDCKNGWKGK TCHSRDSQCD EATCNNGGTC 720
YDEGDAFKCM CPGGWEGTTC NIARNSSCLP NPCHNGGTCV VNGESFTCVC KEGWEGPICA 780
QNTNDCSPHP CYNSGTCVDG DNWYRCECAP GFAGPDCRIN INECQSSPCA FGATCVDEIN 840
GYRCVCPPGH SGAKCQEVSG RPCITMGSVI PDGAKWDDDC NTCQCLNGRI ACSKVWCGPR 900
PCLLHKGHSE CPSGQSCIPI LDDQCFVHPC TGVGECRSSS LQPVKTKCTS DSYYQDNCAN 960
ITFTFNKEMM SPGLTTEHIC SELRNLNILK NVSAEYSIYI ACEPSPSANN EIHVAISAED 1020
IRDDGNPIKE ITDKIIDLVS KRDGNSSLIA AVAEVRVQRR PLKNRTDFLV PLLSSVLTVA 1080
WICCLVTAFY WCLRKRRKPG SHTHSASEDN TTNNVREQLN QIKNPIEKHG ANTVPIKDYE 1140
NKNSKMSKIR THNSEVEEDD MDKHQQKARF AKQPAYTLVD REEKPPNGTP TKHPNWTNKQ 1200
DNRDLESAQS LNRMEYIV 1218 
Gene Ontology
 GO:0045177; C:apical part of cell; IEA:Compara.
 GO:0005576; C:extracellular region; NAS:UniProtKB.
 GO:0005887; C:integral to plasma membrane; NAS:UniProtKB.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0008083; F:growth factor activity; NAS:UniProtKB.
 GO:0005112; F:Notch binding; NAS:UniProtKB.
 GO:0005198; F:structural molecule activity; NAS:UniProtKB.
 GO:0001525; P:angiogenesis; NAS:UniProtKB.
 GO:0035909; P:aorta morphogenesis; ISS:BHF-UCL.
 GO:0042491; P:auditory receptor cell differentiation; IEA:Compara.
 GO:0061309; P:cardiac neural crest cell development involved in outflow tract morphogenesis; ISS:BHF-UCL.
 GO:0003215; P:cardiac right ventricle morphogenesis; ISS:BHF-UCL.
 GO:0060411; P:cardiac septum morphogenesis; ISS:BHF-UCL.
 GO:0001709; P:cell fate determination; NAS:UniProtKB.
 GO:0072017; P:distal tubule development; IEA:Compara.
 GO:0061444; P:endocardial cushion cell development; ISS:BHF-UCL.
 GO:0045446; P:endothelial cell differentiation; NAS:UniProtKB.
 GO:0030097; P:hemopoiesis; NAS:UniProtKB.
 GO:0030216; P:keratinocyte differentiation; NAS:UniProtKB.
 GO:0072070; P:loop of Henle development; IEA:Compara.
 GO:0002011; P:morphogenesis of an epithelial sheet; IEA:Compara.
 GO:0045445; P:myoblast differentiation; NAS:UniProtKB.
 GO:0045599; P:negative regulation of fat cell differentiation; IEA:Compara.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:2000737; P:negative regulation of stem cell differentiation; IMP:UniProtKB.
 GO:0007399; P:nervous system development; NAS:UniProtKB.
 GO:0097150; P:neuronal stem cell maintenance; IEP:UniProtKB.
 GO:0007220; P:Notch receptor processing; TAS:Reactome.
 GO:0061314; P:Notch signaling involved in heart development; IMP:BHF-UCL.
 GO:0045639; P:positive regulation of myeloid cell differentiation; IEA:Compara.
 GO:0045747; P:positive regulation of Notch signaling pathway; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0061156; P:pulmonary artery morphogenesis; IMP:BHF-UCL.
 GO:0003184; P:pulmonary valve morphogenesis; IMP:BHF-UCL.
 GO:0030334; P:regulation of cell migration; NAS:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; NAS:UniProtKB.
 GO:0032495; P:response to muramyl dipeptide; IEA:Compara. 
Interpro
 IPR001774; DSL.
 IPR000742; EG-like_dom.
 IPR001881; EGF-like_Ca-bd.
 IPR013032; EGF-like_CS.
 IPR000152; EGF-type_Asp/Asn_hydroxyl_site.
 IPR018097; EGF_Ca-bd_CS.
 IPR026219; Jagged/Serrate.
 IPR011651; Notch_ligand_N.
 IPR001007; VWF_C. 
Pfam
 PF01414; DSL
 PF00008; EGF
 PF07645; EGF_CA
 PF07657; MNNL 
SMART
 SM00051; DSL
 SM00181; EGF
 SM00179; EGF_CA
 SM00214; VWC 
PROSITE
 PS00010; ASX_HYDROXYL
 PS51051; DSL
 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3
 PS01187; EGF_CA 
PRINTS
 PR02059; JAGGEDFAMILY.