UniProt Accession

 H3BM89_HUMAN; H3BM89 

Genbank Protein ID

 AC116913; CH471082 

Genbank Nucleotide ID

 EAW77773.1 

Protein Name

 60S ribosomal protein L4 

Protein Synonyms/Alias

 Ribosomal protein L4, isoform CRA_a 

Gene Name

 RPL4 

Gene Synonyms/Alias

 hCG_2004318 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
12	GRMFAPTKTWRRWHR	acetylation	[1]
12	GRMFAPTKTWRRWHR	ubiquitination	[2, 3, 4, 5, 6, 7, 8]
46	LPALVMSKGHRIEEV	ubiquitination	[3, 4, 5, 6, 8]
63	LPLVVEDKVEGYKKT	ubiquitination	[3, 4, 5, 6, 7, 8, 9]
68	EDKVEGYKKTKEAVL	ubiquitination	[3, 4, 6, 7, 9]
69	DKVEGYKKTKEAVLL	ubiquitination	[4, 6]
71	VEGYKKTKEAVLLLK	ubiquitination	[3, 4, 5, 6, 7, 8, 9]
78	KEAVLLLKKLKAWND	ubiquitination	[4, 5, 6, 7, 8, 9]
79	EAVLLLKKLKAWNDI	ubiquitination	[4, 6, 8]
81	VLLLKKLKAWNDIKK	ubiquitination	[3, 4, 6, 8, 9]
87	LKAWNDIKKVYASQR	ubiquitination	[3, 4, 6, 8, 9]
88	KAWNDIKKVYASQRM	ubiquitination	[4, 6]
125	NEDNGIIKAFRNIPG	ubiquitination	[2, 3, 4, 6, 8, 9]
140	ITLLNVSKLNILKLA	ubiquitination	[2, 3, 4, 6, 7, 8]
145	VSKLNILKLAPGGHV	ubiquitination	[3, 4, 6, 8, 9]
165	WTESAFRKLDELYGT	ubiquitination	[3, 6, 8, 9]
175	ELYGTWRKAASLKSN	ubiquitination	[4, 6, 8]
180	WRKAASLKSNYNLPM	ubiquitination	[2, 3, 4, 6, 7, 8, 9]
189	NYNLPMHKMINTDLS	ubiquitination	[2, 3, 4, 5, 6, 7, 8, 9]
200	TDLSRILKSPEIQRA	ubiquitination	[2, 3, 4, 5, 6, 7, 8, 9]
221	KIHRRVLKKNPLKNL	ubiquitination	[6]
222	IHRRVLKKNPLKNLR	ubiquitination	[4]
226	VLKKNPLKNLRIMLK	ubiquitination	[3, 4, 6, 7, 8]
233	KNLRIMLKLNPYAKT	ubiquitination	[2, 3, 4, 5, 6, 7, 8, 9]
239	LKLNPYAKTMRRNTI	ubiquitination	[2, 3, 4, 5, 6, 7, 8, 9]
259	NHKLRVDKAAAAAAA	ubiquitination	[2, 3, 4, 5, 6, 7, 8, 9]
270	AAAALQAKSDEKAAV	acetylation	[10]
270	AAAALQAKSDEKAAV	ubiquitination	[4, 5, 6, 7, 8, 9]
274	LQAKSDEKAAVAGKK	acetylation	[8]
274	LQAKSDEKAAVAGKK	ubiquitination	[4, 5, 7, 8, 9]
280	EKAAVAGKKPVVGKK	ubiquitination	[4, 6, 8]

Reference

 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome; Ribonucleoprotein; Ribosomal protein. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 333 AA 

Protein Sequence

Gene Ontology

 GO:0005840; C:ribosome; IEA:UniProtKB-KW.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0006412; P:translation; IEA:InterPro. 

Interpro

 IPR025755; Ribos_L4_C_dom.
 IPR002136; Ribosomal_L4/L1e.
 IPR013000; Ribosomal_L4/L1e_euk/arc_CS.
 IPR023574; Ribosomal_L4_dom. 

Pfam

 PF14374; Ribos_L4_asso_C
 PF00573; Ribosomal_L4 

SMART

  

PROSITE

 PS00939; RIBOSOMAL_L1E 

PRINTS